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Literature summary extracted from

  • Jiang, X.; Chen, P.; Yin, M.; Yang, Q.
    Constitutive expression, purification and characterisation of pectin methylesterase from Aspergillus niger in Pichia pastoris for potential application in the fruit juice industry (2013), J. Sci. Food Agric., 93, 375-381.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.11 expressed in Pichia pastoris strain GS115 Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.11 food industry fruit juice industry Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.11 Co2+ 52% residual activity at 100 mM Aspergillus niger
3.1.1.11 K+ 73% residual activity at 100 mM Aspergillus niger
3.1.1.11 Mg2+ 86% residual activity at 100 mM Aspergillus niger
3.1.1.11 Mn2+ 61% residual activity at 100 mM Aspergillus niger
3.1.1.11 Ni2+ 47% residual activity at 100 mM Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.11 additional information not affected by Na+ Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.11 45000
-
 x * 45000, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.11 pectin + H2O Aspergillus niger
-
 methanol + pectate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.11 Aspergillus niger
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.11 glycoprotein
-
 Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.11 ammonium sulfate precipitation, HiTrap Q column chromatography, and Resource S column chromatography Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.11 9.67
-
 supernatant, at pH 4.7 and 50°C Aspergillus niger
3.1.1.11 342
-
 after 35.4fold purification, at pH 4.7 and 50°C Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.11 pectin + H2O
-
 Aspergillus niger methanol + pectate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.11 ? x * 45000, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.1.1.11 pectin methylesterase
-
 Aspergillus niger
3.1.1.11 PME
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.11 50
-
-
 Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.11 35 60 90-100% activity from 35-50°C, about 30% activity at 55°C, no activity at 60°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.11 4.7
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.11 4.5 5 about 90% activity at pH 4.5,4.6, and 4.8, 100% activity at pH 4.7, about 60% activity at pH 4.9, about 50% activity at pH 5.0 Aspergillus niger