Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tsuchida, S.; Kawamoto, K.; Endo, N.; Nunome, K.; Hamaue, N.; Aoki, T.
    Hydratase activities of green fluorescent protein tagged human multifunctional enzyme type 2 hydratase domain and its variants (2012), J. Oleo Sci., 61, 443-450.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.119 expression of wild-type and mutant hydratase domains of multifunctional enzyme type 2 hydratase as GFP-tagged protein in Escherichia coli strain JM109 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.119 A348T site-directed mutagenesis, the mutation does not affect the enzyme Homo sapiens
4.2.1.119 A427V site-directed mutagenesis, the mutation does not affect the enzyme, the mutant shows a slight increase in activity Homo sapiens
4.2.1.119 A491T site-directed mutagenesis, the mutation does not affect the enzyme Homo sapiens
4.2.1.119 A606S site-directed mutagenesis, the mutant shows reduced activity Homo sapiens
4.2.1.119 D510Y site-directed mutagenesis, inactive mutant, the mutation disrupts active site architecture Homo sapiens
4.2.1.119 E366G site-directed mutagenesis, inactive mutant, the mutation disrupts dimerization Homo sapiens
4.2.1.119 G533R site-directed mutagenesis, inactive mutant, the mutation disrupts ligand interaction Homo sapiens
4.2.1.119 H532R site-directed mutagenesis, inactive mutant, the mutation disrupts active site architecture Homo sapiens
4.2.1.119 I516T site-directed mutagenesis, the mutation disrupts dimerization, the mutant shows reduced activity Homo sapiens
4.2.1.119 I559V site-directed mutagenesis, the mutation does not affect the enzyme Homo sapiens
4.2.1.119 L405P site-directed mutagenesis, inactive mutant, the mutation disrupts ligand interaction Homo sapiens
4.2.1.119 additional information site-directed mutagenesis is used for construction of mutants corresponding to 17 reported missense mutations of multifunctional enzyme type 2 hydratase. Some mutants are almost or completely inactive causing the D-bifunctional protein deficiency type II Homo sapiens
4.2.1.119 N457D site-directed mutagenesis, the mutant shows reduced activity Homo sapiens
4.2.1.119 N457Y site-directed mutagenesis, the mutation disrupts domain folding, the mutant shows reduced activity Homo sapiens
4.2.1.119 P529L site-directed mutagenesis, inactive mutant, the mutation disrupts active site architecture Homo sapiens
4.2.1.119 R506C site-directed mutagenesis, inactive mutant, the mutation disrupts dimerization Homo sapiens
4.2.1.119 R506H site-directed mutagenesis, inactive mutant, the mutation disrupts dimerization Homo sapiens
4.2.1.119 W511R site-directed mutagenesis, the mutation does not affect the enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.119 peroxisome
-
 Homo sapiens 5777
-

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.119 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.119 recombinant wild-type and mutant GFP-tagged hydratase domains of multifunctional enzyme type 2 hydratase from Escherichia coli strain JM109 by anion exchange chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.119 (3R)-3-hydroxyhexadecanoyl-CoA
-
 Homo sapiens (2E)-2-hexadecenoyl-CoA + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.119 MFE2 hydratase
-
 Homo sapiens
4.2.1.119 multifunctional enzyme type 2 hydratase
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
4.2.1.119 malfunction inactivating mutations of multifunctional enzyme type 2 hydratase lead to D-bifunctional protein deficiency type II Homo sapiens
4.2.1.119 additional information MFE2 hydratase is R-specific, while MFE1 hydratase is S-specific Homo sapiens