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Literature summary extracted from

  • Kurokawa, S.; Takehashi, M.; Tanaka, H.; Mihara, H.; Kurihara, T.; Tanaka, S.; Hill, K.; Burk, R.; Esaki, N.
    Mammalian selenocysteine lyase is involved in selenoprotein biosynthesis (2011), J. Nutr. Sci. Vitaminol., 57, 298-305.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.16 recombinant expression in Escherichia coli strain BL21(DE3) Rattus norvegicus
4.4.1.16 recombinant expression of GFP-tagged enzyme in spermatids of mature testes of transgenic mice Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.16 additional information HeLa cells transfected with enzyme-specific siRNA show decreased seleno-labeling efficiency of selenoproteins with selenoprotein P compared to the wild-type Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.4.1.16 nucleus
-
 Mus musculus 5634
-
4.4.1.16 nucleus
-
 Homo sapiens 5634
-
4.4.1.16 nucleus
-
 Rattus norvegicus 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.16 L-selenocysteine + reduced acceptor Mus musculus
-
 selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor Homo sapiens
-
 selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor Rattus norvegicus
-
 selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor Mus musculus C57/BL6
-
 selenide + L-alanine + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.16 Homo sapiens
-
-
-
4.4.1.16 Mus musculus
-
-
-
4.4.1.16 Mus musculus C57/BL6
-
-
-
4.4.1.16 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.16 HeLa cell
-
 Homo sapiens
-
4.4.1.16 kidney
-
 Rattus norvegicus
-
4.4.1.16 kidney high expression level in nuclei of renal proximal tubules, less in distal tubules, no expression in glomerulus cells Mus musculus
-
4.4.1.16 Leydig cell
-
 Mus musculus
-
4.4.1.16 Leydig cell
-
 Homo sapiens
-
4.4.1.16 Leydig cell
-
 Rattus norvegicus
-
4.4.1.16 liver
-
 Mus musculus
-
4.4.1.16 liver
-
 Rattus norvegicus
-
4.4.1.16 additional information tissue expression pattern, cell-specific expression in kidney and liver, overview Mus musculus
-
4.4.1.16 seminiferous tubule dominantly in Leydig cells and spermatids Mus musculus
-
4.4.1.16 seminiferous tubule dominantly in Leydig cells and spermatids Homo sapiens
-
4.4.1.16 seminiferous tubule dominantly in Leydig cells and spermatids Rattus norvegicus
-
4.4.1.16 Sertoli cell low enzyme level Mus musculus
-
4.4.1.16 spermatid
-
 Homo sapiens
-
4.4.1.16 spermatid
-
 Rattus norvegicus
-
4.4.1.16 spermatid high enzyme content and activity Mus musculus
-
4.4.1.16 testis
-
 Mus musculus
-
4.4.1.16 testis
-
 Homo sapiens
-
4.4.1.16 testis
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Mus musculus selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Homo sapiens selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Rattus norvegicus selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Mus musculus C57/BL6 selenide + L-alanine + acceptor
-
 ?
4.4.1.16 additional information the enzyme is specific for L-selenocysteine versus L-cysteine on a molecular level, no activity with L-cysteine Mus musculus ?
-
 ?
4.4.1.16 additional information the enzyme is specific for L-selenocysteine versus L-cysteine on a molecular level, no activity with L-cysteine Homo sapiens ?
-
 ?
4.4.1.16 additional information the enzyme is specific for L-selenocysteine versus L-cysteine on a molecular level, no activity with L-cysteine. Residue Cys375 sorts selenium from cysteine and directs it to the active site Rattus norvegicus ?
-
 ?
4.4.1.16 additional information the enzyme is specific for L-selenocysteine versus L-cysteine on a molecular level, no activity with L-cysteine Mus musculus C57/BL6 ?
-
 ?

Expression

EC Number Organism Comment Expression
4.4.1.16 Homo sapiens presence or absence of selenoproteins does not affect enzyme expression in HeLa cells additional information

General Information

EC Number General Information Comment Organism
4.4.1.16 additional information the enzyme is involved in selenocysteine recovering from selenoproteins to be used for synthesis of new selenoproteins Rattus norvegicus