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Literature summary extracted from
- Structural analysis of the Leptospiral sphingomyelinases: in silico and experimental evaluation of Sph2 as an Mg++-dependent sphingomyelinase (2012), J. Mol. Microbiol. Biotechnol., 22, 24-34.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.12 | gene sph2, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strains DH5alpha, recombinant expression in Escherichia coli strain BL21(DE3) in inclusion bodies | Leptospira interrogans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | Ca2+ | inhibits the hemolytic enzyme activity | Leptospira interrogans |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | Mg2+ | dependent on, isozyme Sph2 is unique and possesses the Mg2+-binding Glu53 residue in the metal-binding site | Leptospira interrogans | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | a sphingomyelin + H2O | Leptospira interrogans | - |
a ceramide + phosphocholine | a ceramide is an N-acylsphingosine | ? | |
| 3.1.4.12 | a sphingomyelin + H2O | Leptospira interrogans 56601 | - |
a ceramide + phosphocholine | a ceramide is an N-acylsphingosine | ? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | Leptospira interrogans | P59116 | serovar Lai, gene sph2 or LA_1029 encoding isozyme Sph2 | - |
| 3.1.4.12 | Leptospira interrogans 56601 | P59116 | serovar Lai, gene sph2 or LA_1029 encoding isozyme Sph2 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.12 | recombinant solubilized enzyme from Escherichia coli strain BL21(DE3) inclusion bodies by immunoaffinity chromatography | Leptospira interrogans |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.1.4.12 | recombinant enzyme from Escherichia coli strain BL21(DE3) inclusion bodies is solubilized by 8 M urea | Leptospira interrogans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | a sphingomyelin + H2O | - |
Leptospira interrogans | a ceramide + phosphocholine | a ceramide is an N-acylsphingosine | ? | |
| 3.1.4.12 | a sphingomyelin + H2O | - |
Leptospira interrogans 56601 | a ceramide + phosphocholine | a ceramide is an N-acylsphingosine | ? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | Mg++-dependent sphingomyelinase | - |
Leptospira interrogans |
| 3.1.4.12 | Sph2 | - |
Leptospira interrogans |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | evolution | the enzyme belongs to the DNase I superfamily, which contains the exo-endo-phosphatase domain, the domain lacks only in isozyme Sph4, all isozymes lack the beta-hairpin loop, the organism encodes genes Sph1, Sph2, Sph3, Sph4 and SphH, structure comparisons, overview | Leptospira interrogans |
| 3.1.4.12 | additional information | isozyme Sph2 structure homology modeling, the isozyme Sph2 is unique and possesses the Mg2+-binding Glu53 residue in the metal-binding site and two His residues, His151 and His286, in the catalytic site | Leptospira interrogans |
| 3.1.4.12 | physiological function | the recombinant isozyme Sph2 performs the Mg2+ -dependent hemolysis of erythrocytes | Leptospira interrogans |
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