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Literature summary extracted from

  • Izumikawa, M.; Shipley, P.R.; Hopke, J.N.; OHare, T.; Xiang, L.; Noel, J.P.; Moore, B.S.
    Expression and characterization of the type III polyketide synthase 1,3,6,8-tetrahydroxynaphthalene synthase from Streptomyces coelicolor A3(2) (2003), J. Ind. Microbiol. Biotechnol., 30, 510-515.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.233 expressed in Escherichia coli BL21(DE3)pLysS cells Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.233 0.00358
-
 malonyl-CoA at pH 7.5 and 22°C Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.233 43000
-
 x * 43000, SDS-PAGE Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.233 5 malonyl-CoA Streptomyces coelicolor
-
 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
 ?
2.3.1.233 5 malonyl-CoA Streptomyces coelicolor A3(2)
-
 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.233 Streptomyces coelicolor Q9FCA7
-
-
2.3.1.233 Streptomyces coelicolor A3(2) Q9FCA7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.233 Ni2+-affinity column chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.233 5 malonyl-CoA
-
 Streptomyces coelicolor 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
 ?
2.3.1.233 5 malonyl-CoA
-
 Streptomyces coelicolor A3(2) 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.233 ? x * 43000, SDS-PAGE Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
2.3.1.233 1,3,6,8-tetrahydroxynaphthalene synthase
-
 Streptomyces coelicolor
2.3.1.233 PKS
-
 Streptomyces coelicolor
2.3.1.233 SCO1206 locus name Streptomyces coelicolor
2.3.1.233 THNS
-
 Streptomyces coelicolor
2.3.1.233 type III polyketide synthase
-
 Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.233 0.008
-
 malonyl-CoA at pH 7.5 and 22°C Streptomyces coelicolor