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Literature summary extracted from
- Production of a periplasmic trehalase in Gluconobacter oxydans and growth on trehalose (2014), J. Biotechnol., 189C, 27-35.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.28 | expressed in Gluconobacter oxydans | Escherichia coli |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.28 | periplasm | - |
Escherichia coli | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 61000 | - |
x * 61000, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.28 | alpha,alpha-trehalose + H2O | Escherichia coli | - |
beta-D-glucose + alpha-D-glucose | - |
? |  |
| 3.2.1.28 | alpha,alpha-trehalose + H2O | Escherichia coli K12 DH5alpha | - |
beta-D-glucose + alpha-D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.28 | Escherichia coli | - |
- |
- |
| 3.2.1.28 | Escherichia coli K12 DH5alpha | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 0.5 | - |
enzyme in periplasmic fraction, pH and temperature not specified in the publication | Escherichia coli |
| 3.2.1.28 | 6.2 | - |
enzyme in periplasmic fraction, pH and temperature not specified in the publication | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.28 | alpha,alpha-trehalose + H2O | - |
Escherichia coli | beta-D-glucose + alpha-D-glucose | - |
? | |
| 3.2.1.28 | alpha,alpha-trehalose + H2O | - |
Escherichia coli K12 DH5alpha | beta-D-glucose + alpha-D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.28 | ? | x * 61000, SDS-PAGE | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.28 | TreA | - |
Escherichia coli |
| 3.2.1.28 | trehalase | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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