EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.20 | expressed in Pichia pastoris strain RIB40 | Aspergillus oryzae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.20 | 0.23 | - |
epicatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 0.26 | - |
epigallocatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 0.32 | - |
epigallocatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 0.34 | - |
epicatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 0.45 | - |
catechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 0.47 | - |
catechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 0.86 | - |
ethyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 1.02 | - |
methyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 1.12 | - |
propyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 1.3 | - |
ethyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 1.38 | - |
propyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 1.97 | - |
methyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.20 | tannic acid + H2O | Aspergillus oryzae | - |
gallate + D-glucose | - |
? | |
3.1.1.20 | tannic acid + H2O | Aspergillus oryzae RIB 40 | - |
gallate + D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.20 | Aspergillus oryzae | - |
- |
- |
3.1.1.20 | Aspergillus oryzae RIB 40 | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.1.20 | glycoprotein | - |
Aspergillus oryzae |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.20 | TSKgel DEAE-5PW column chromatography and G3000-SWXL gel filtration | Aspergillus oryzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.20 | catechin gallate + H2O | - |
Aspergillus oryzae | gallate + catechin | - |
? | |
3.1.1.20 | epicatechin gallate + H2O | best substrate | Aspergillus oryzae | gallate + epicatechin | - |
? | |
3.1.1.20 | epicatechin gallate + H2O | best substrate | Aspergillus oryzae RIB 40 | gallate + epicatechin | - |
? | |
3.1.1.20 | epigallocatechin gallate + H2O | - |
Aspergillus oryzae | gallate + epigallocatechin | - |
? | |
3.1.1.20 | ethyl gallate + H2O | worst substrate | Aspergillus oryzae | gallate + ethanol | - |
? | |
3.1.1.20 | methyl gallate + H2O | - |
Aspergillus oryzae | gallate + methanol | - |
? | |
3.1.1.20 | methyl gallate + H2O | - |
Aspergillus oryzae RIB 40 | gallate + methanol | - |
? | |
3.1.1.20 | additional information | no activities against the methyl esters of ferulic, 4-coumaric, caffeic, and sinapic acids, or the ethyl, propyl, and butyl esters of 4-hydroxybenzoic acid | Aspergillus oryzae | ? | - |
? | |
3.1.1.20 | additional information | no activities against the methyl esters of ferulic, 4-coumaric, caffeic, and sinapic acids, or the ethyl, propyl, and butyl esters of 4-hydroxybenzoic acid | Aspergillus oryzae RIB 40 | ? | - |
? | |
3.1.1.20 | propyl gallate + H2O | - |
Aspergillus oryzae | gallate + propanol | - |
? | |
3.1.1.20 | propyl gallate + H2O | - |
Aspergillus oryzae RIB 40 | gallate + propanol | - |
? | |
3.1.1.20 | tannic acid + H2O | - |
Aspergillus oryzae | gallate + D-glucose | - |
? | |
3.1.1.20 | tannic acid + H2O | - |
Aspergillus oryzae RIB 40 | gallate + D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.20 | ? | x * 30000-33000, deglycosylated native enzyme, SDS-PAGE | Aspergillus oryzae |
3.1.1.20 | ? | x * 30000-34000, deglycosylated recombinant enzyme, SDS-PAGE | Aspergillus oryzae |
3.1.1.20 | ? | x * 45000-75000, recombinant enzyme, smeared bands on SDS-PAGE | Aspergillus oryzae |
3.1.1.20 | ? | x * 45000-80000, native enzyme, smeared bands on SDS-PAGE | Aspergillus oryzae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.20 | TanA | - |
Aspergillus oryzae |
3.1.1.20 | tannin acyl hydrolase | - |
Aspergillus oryzae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.20 | 40 | 50 | the enzyme is stable up to 40°C. Native enzyme retains more than 70% of ist activity after incubation at 50°C for 60 min, whereas the residual activity of recombinant enzyme is approximately 40% after treatment at 50°C for 60 min | Aspergillus oryzae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.20 | 9.8 | - |
ethyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 18.6 | - |
propyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 19 | - |
ethyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 20.5 | - |
propyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 60.2 | - |
methyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 62.1 | - |
epigallocatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 64.5 | - |
catechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 67.5 | - |
epicatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 70.2 | - |
methyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 79.8 | - |
catechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 86.1 | - |
epigallocatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 103.4 | - |
epicatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.20 | 4 | 5 | native enzyme | Aspergillus oryzae |
3.1.1.20 | 5 | - |
purified enzyme | Aspergillus oryzae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.20 | 7.54 | - |
ethyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 14.9 | - |
propyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 16.6 | - |
propyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 22.1 | - |
ethyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 30.6 | - |
methyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 69.2 | - |
methyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 137.3 | - |
catechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 179.3 | - |
catechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 234 | - |
epigallocatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 273.4 | - |
epigallocatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 293.6 | - |
epicatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
3.1.1.20 | 308.8 | - |
epicatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae |