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Literature summary extracted from
- An ancient relative of cyclooxygenase in cyanobacteria is a linoleate 10S-dioxygenase that works in tandem with a catalase-related protein with specific 10S-hydroperoxide lyase activity (2014), J. Biol. Chem., 289, 13101-13111.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.92 | gene Npun_R5468, expression of the enzyme with N- or C-terminal His6 tags in Escherichia coli strain BL21(DE3) | Nostoc punctiforme |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.92 | 45000 | - |
x * 45000, about, sequence calculation | Nostoc punctiforme |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.92 | Nostoc punctiforme | B2J5P3 | gene Npun_R5468 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.92 | recombinant N- or C-terminal His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Nostoc punctiforme |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.92 | (8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoic acid | the catalase-related hemoprotein reacts rapidly and specifically with linoleate 10S-hydroperoxide with a hydroperoxide lyase activity specific for the 10S-hydroperoxy enantiomer | Nostoc punctiforme | (8E)-10-oxodecenoic acid + 1-octen-3-ol + (2Z)-octen-1-ol | in a 3:1 ratio, strict enzymatic control in formation of the 3R alcohol configuration with 99% enantiomeric excess | ? |  |
| 4.2.1.92 | additional information | the enzyme mainly acts as an oleate or linoleate dioxygenase, albeit with alpha-linolenic acid very competitive at low substrate concentrations, substrate specificity and kinetics, overview | Nostoc punctiforme | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.92 | ? | x * 45000, about, sequence calculation | Nostoc punctiforme |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.92 | 10S-hydroperoxide lyase | - |
Nostoc punctiforme |
| 4.2.1.92 | Npun_R5469 | - |
Nostoc punctiforme |
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Release 2023.1 (January 2023)
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