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Literature summary extracted from

  • Baba, T.; Kashiwagi, Y.; Arimitsu, N.; Kogure, T.; Edo, A.; Maruyama, T.; Nakao, K.; Nakanishi, H.; Kinoshita, M.; Frohman, M.A.; Yamamoto, A.; Tani, K.
    Phosphatidic acid (PA)-preferring phospholipase A1 regulates mitochondrial dynamics (2014), J. Biol. Chem., 289, 11497-11511.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.32 expression of FLAG-tagged wild-type and mutant enzymes in HeLa cells and in siRNA-treated knockout mutant HeLa cells. Upon overexpression of wild-type enzyme, the tubular structures become fragmented, expression of the mutant S537A has no effect Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.32 additional information construction of enzyme knockout mutant HeLa cells by treatment with specific siRNA, phenotype, overview Homo sapiens
3.1.1.32 additional information generation of phosphatidic acid-preferring phospholipase A1 knock-out mice using targeting vector clone PGS00049_B_D06 and specific siRNA, phenotype, overview Mus musculus
3.1.1.32 S537A site-directed mutagenesis, inactive mutant Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.32 intracellular
-
 Mus musculus 5622
-
3.1.1.32 intracellular
-
 Homo sapiens 5622
-
3.1.1.32 mitochondrion
-
 Mus musculus 5739
-
3.1.1.32 mitochondrion
-
 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.32 phosphatidic acid + H2O Mus musculus
-
 2-acyl-lysophosphatidic acid + a carboxylate
-
 ?
3.1.1.32 phosphatidic acid + H2O Homo sapiens
-
 2-acyl-lysophosphatidic acid + a carboxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.32 Homo sapiens
-
-
-
3.1.1.32 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.32 brain high expression level Homo sapiens
-
3.1.1.32 caput epididymis
-
 Mus musculus
-
3.1.1.32 cauda epididymis
-
 Mus musculus
-
3.1.1.32 HeLa cell
-
 Homo sapiens
-
3.1.1.32 spermatozoon
-
 Mus musculus
-
3.1.1.32 testis high expression level Mus musculus
-
3.1.1.32 testis high expression level Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.32 phosphatidic acid + H2O
-
 Mus musculus 2-acyl-lysophosphatidic acid + a carboxylate
-
 ?
3.1.1.32 phosphatidic acid + H2O
-
 Homo sapiens 2-acyl-lysophosphatidic acid + a carboxylate
-
 ?
3.1.1.32 phosphatidic acid + H2O preferred substrate in vitro Mus musculus 2-acyl-lysophosphatidic acid + a carboxylate
-
 ?
3.1.1.32 phosphatidic acid + H2O preferred substrate in vitro Homo sapiens 2-acyl-lysophosphatidic acid + a carboxylate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.32 PA-PLA1/DDHD1
-
 Mus musculus
3.1.1.32 PA-PLA1/DDHD1
-
 Homo sapiens
3.1.1.32 phosphatidic acid-preferring phospholipase A1
-
 Mus musculus
3.1.1.32 phosphatidic acid-preferring phospholipase A1
-
 Homo sapiens
3.1.1.32 phosphatidic acid-preferring phospholipase A1/DDHD1
-
 Mus musculus
3.1.1.32 phosphatidic acid-preferring phospholipase A1/DDHD1
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
3.1.1.32 malfunction phosphatidic acid-preferring phospholipase A1 depletion causes mitochondrial elongation, leading to a loss of motility Homo sapiens
3.1.1.32 malfunction phosphatidic acid-preferring phospholipase A1 depletion causes mitochondrial elongation. Enzyme knock-out mice have a defect in sperm formation, spermatozoa from PA-PLA1-/- mice possess an annulus that is not attached to the mitochondrial sheath, whereas that in control mice is attached. In enzyme-deficient sperm, the mitochondrial structure is disorganized, and an abnormal gap structure exists between the middle and principal pieces.A flagellum is bent at that position, leading to a loss of motility Mus musculus
3.1.1.32 physiological function the phosphatidic acid-preferring phospholipase A1 regulates mitochondrial dynamics Mus musculus
3.1.1.32 physiological function the phosphatidic acid-preferring phospholipase A1 regulates mitochondrial dynamics. When ectopically expressed in HeLa cells, it induces mitochondrial fragmentation, whereas its depletion causes mitochondrial elongation. The effects of the enzyme on mitochondrial morphology appear to counteract those of MitoPLD, a mitochondrion-localized phospholipase D that produces phosphatidic acid from cardiolipin Homo sapiens