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Literature summary extracted from

  • Austin, M.B.; Izumikawa, M.; Bowman, M.E.; Udwary, D.W.; Ferrer, J.L.; Moore, B.S.; Noel, J.P.
    Crystal structure of a bacterial type III polyketide synthase and enzymatic control of reactive polyketide intermediates (2004), J. Biol. Chem., 279, 45162-45174.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.233 expressed in Escherichia coli BL21(DE3) cells Streptomyces coelicolor

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.233 hanging drop vapor diffusion method, using 14% (w/v) PEG 8000, 200 mM MgCl2, 100 mM Na-MOPSO buffer (pH 7.0), 5 mM dithiothreitol, and 3% (w/v) sucrose Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.233 C106S inactive Streptomyces coelicolor
2.3.1.233 C171S the mutant shows about 4fold reduced catalytic efficiency compared to the wild type enzyme Streptomyces coelicolor
2.3.1.233 C184S the mutant shows about 2.5fold increased catalytic efficiency compared to the wild type enzyme Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.233 0.0014
-
 malonyl-CoA mutant enzyme C184S, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor
2.3.1.233 0.0023
-
 malonyl-CoA wild type enzyme, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor
2.3.1.233 0.0085
-
 malonyl-CoA mutant enzyme C171S, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.233 5 malonyl-CoA Streptomyces coelicolor
-
 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.233 Streptomyces coelicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.233
-
 Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.233 5 malonyl-CoA
-
 Streptomyces coelicolor 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.233 1,3,6,8-tetrahydroxynaphthalene synthase
-
 Streptomyces coelicolor
2.3.1.233 PKS
-
 Streptomyces coelicolor
2.3.1.233 THNS
-
 Streptomyces coelicolor
2.3.1.233 type III polyketide synthase
-
 Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.233 45.7
-
 malonyl-CoA wild type enzyme, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor
2.3.1.233 46.5
-
 malonyl-CoA mutant enzyme C171S, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor
2.3.1.233 79.2
-
 malonyl-CoA mutant enzyme C184S, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.233 0.55
-
 malonyl-CoA mutant enzyme C171S, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor
2.3.1.233 1.97
-
 malonyl-CoA wild type enzyme, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor
2.3.1.233 5.53
-
 malonyl-CoA mutant enzyme C184S, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor