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Literature summary extracted from

  • Stratmann, A.; Mahmud, T.; Lee, S.; Distler, J.; Floss, H.G.; Piepersberg, W.
    The AcbC protein from Actinoplanes species is a C7-cyclitol synthase related to 3-dehydroquinate synthases and is involved in the biosynthesis of the alpha-glucosidase inhibitor acarbose (1999), J. Biol. Chem., 274, 10889-10896.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.152 overexpressed heterologously in Streptomyces lividans 66 Actinoplanes sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.152 Co2+ assay bufer contains Co2+ Actinoplanes sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.152 D-sedoheptulose 7-phosphate Actinoplanes sp. the cyclization product, 2-epi-5-epi-valiolone is a precursor of the valienamine moiety of acarbose 2-epi-5-epi-valiolone + phosphate
-
 ?
4.2.3.152 D-sedoheptulose 7-phosphate Actinoplanes sp. 50/110 the cyclization product, 2-epi-5-epi-valiolone is a precursor of the valienamine moiety of acarbose 2-epi-5-epi-valiolone + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.152 Actinoplanes sp. Q9ZAE9
-
-
4.2.3.152 Actinoplanes sp. 50/110 Q9ZAE9
-
-

Storage Stability

EC Number Storage Stability Organism
4.2.3.152 -20°C, AcbC extract can be stored for 3 months without major loss of activity Actinoplanes sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.152 D-sedoheptulose 7-phosphate the cyclization product, 2-epi-5-epi-valiolone is a precursor of the valienamine moiety of acarbose Actinoplanes sp. 2-epi-5-epi-valiolone + phosphate
-
 ?
4.2.3.152 D-sedoheptulose 7-phosphate no activity with ido-heptulose 7-phosphate Actinoplanes sp. 2-epi-5-epi-valiolone + phosphate
-
 ?
4.2.3.152 D-sedoheptulose 7-phosphate the cyclization product, 2-epi-5-epi-valiolone is a precursor of the valienamine moiety of acarbose Actinoplanes sp. 50/110 2-epi-5-epi-valiolone + phosphate
-
 ?
4.2.3.152 D-sedoheptulose 7-phosphate no activity with ido-heptulose 7-phosphate Actinoplanes sp. 50/110 2-epi-5-epi-valiolone + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.152 AbcC
-
 Actinoplanes sp.
4.2.3.152 C7-cyclitol synthase
-
 Actinoplanes sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.152 30
-
 assay at Actinoplanes sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.152 7.5
-
 assay at Actinoplanes sp.

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.3.152 NAD+ assay buffer contains NAD+ Actinoplanes sp.