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Literature summary extracted from

  • Austin, C.M.; Maier, R.J.
    Aconitase-mediated posttranscriptional regulation of Helicobacter pylori peptidoglycan deacetylase (2013), J. Bacteriol., 195, 5316-5322.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.104 gene pgdA, in the wild type, pgdAmRNAhalf-life is 13 min, whereas the half-life for the acnB strain is 7 min, apo-AcnB binds to the 3'-untranslated region of the pgdA RNA transcript. AcnB-pgdA transcript binding is abolished by the addition of iron. Real-time quantitative PCR enzyme expression analysis and RNA footprinting Helicobacter pylori
4.2.1.3 expressed in Escherichia coli Helicobacter pylori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.104 cell wall
-
 Helicobacter pylori 5618
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.104 Fe2+ abolishes the acnB-pgdA transcript binding leading to destabilization of the enzyme's mRNA Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.104 peptidoglycan-N-acetyl-D-glucosamine + H2O Helicobacter pylori peptidoglycan consists of alternating N-acetylglucosamine and N-acetylmuramic acid residues connected by beta-1,4 bonds and cross-linked via short peptide bridges peptidoglycan-D-glucosamine + acetate
-
 ?
3.5.1.104 peptidoglycan-N-acetyl-D-glucosamine + H2O Helicobacter pylori ATCC 700392 peptidoglycan consists of alternating N-acetylglucosamine and N-acetylmuramic acid residues connected by beta-1,4 bonds and cross-linked via short peptide bridges peptidoglycan-D-glucosamine + acetate
-
 ?
4.2.1.3 additional information Helicobacter pylori using electrophoretic mobility shift assays and RNA footprinting it is shown that apo-AcnB binds to the 3'-untranslated region of the pgdA RNA transcript ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.104 Helicobacter pylori O25080
-
-
3.5.1.104 Helicobacter pylori ATCC 700392 O25080
-
-
4.2.1.3 Helicobacter pylori
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.104 peptidoglycan-N-acetyl-D-glucosamine + H2O
-
 Helicobacter pylori peptidoglycan-D-glucosamine + acetate
-
 ?
3.5.1.104 peptidoglycan-N-acetyl-D-glucosamine + H2O peptidoglycan consists of alternating N-acetylglucosamine and N-acetylmuramic acid residues connected by beta-1,4 bonds and cross-linked via short peptide bridges Helicobacter pylori peptidoglycan-D-glucosamine + acetate
-
 ?
3.5.1.104 peptidoglycan-N-acetyl-D-glucosamine + H2O
-
 Helicobacter pylori ATCC 700392 peptidoglycan-D-glucosamine + acetate
-
 ?
3.5.1.104 peptidoglycan-N-acetyl-D-glucosamine + H2O peptidoglycan consists of alternating N-acetylglucosamine and N-acetylmuramic acid residues connected by beta-1,4 bonds and cross-linked via short peptide bridges Helicobacter pylori ATCC 700392 peptidoglycan-D-glucosamine + acetate
-
 ?
4.2.1.3 additional information using electrophoretic mobility shift assays and RNA footprinting it is shown that apo-AcnB binds to the 3'-untranslated region of the pgdA RNA transcript Helicobacter pylori ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.104 peptidoglycan deacetylase
-
 Helicobacter pylori
3.5.1.104 pgdA
-
 Helicobacter pylori
4.2.1.3 AcnB
-
 Helicobacter pylori
4.2.1.3 aconitase B
-
 Helicobacter pylori

Expression

EC Number Organism Comment Expression
3.5.1.104 Helicobacter pylori PgdA is highly expressed under oxidative stress up

General Information

EC Number General Information Comment Organism
3.5.1.104 physiological function Helicobacter pylori is equipped with a peptidoglycan deacetylase (PgdA) that confers both pure peptidoglycan and whole bacterial resistance to lysozyme degradation, which hydrolyzes the beta-1,4 bonds in peptidoglycan. Under oxidative stress, PgdA is highly expressed and confers resistance to lysozyme in wild-type cells. Role of aconitase (AcnB) in Helicobacter pylori as a posttranscriptional regulator of the cell wall-modifying enzyme peptidoglycan deacetylase PgdA, apo-AcnB directly interacts with the pgdA transcript to enhance stability and increase deacetylase enzyme expression, which impacts in vivo survival Helicobacter pylori
4.2.1.3 malfunction cell wall-modifying enzyme peptidoglycan deacetylase, PgdA expression is significantly decreased in an acnB mutant. Mutant strain is more susceptible to lysozyme-mediated killing and is attenuated in its ability to colonize mice Helicobacter pylori