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Literature summary extracted from

  • Stolzenberger, J.; Lindner, S.N.; Persicke, M.; Brautaset, T.; Wendisch, V.F.
    Characterization of fructose 1,6-bisphosphatase and sedoheptulose 1,7-bisphosphatase from the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus (2013), J. Bacteriol., 195, 5112-5122.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.3.11 synthesis the substrate for the sedoheptulose 1,7-bisphosphatase reaction, sedoheptulose 1,7-bisphosphate, can be synthesized in vitro by using both the chromosomal-encoded or the plasmid-encoded fructose 1,6-bisphosphate aldolase proteins from Bacillus methanolicus Bacillus methanolicus
3.1.3.11 synthesis the substrate for the sedoheptulose 1,7-bisphosphatase reaction, sedoheptulose 1,7-bisphosphate, can be synthesized in vitro by using both the chromosomal-encoded or theplasmid-encoded fructose 1,6-bisphosphate aldolase proteins from Bacillus methanolicus Bacillus methanolicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.11 expression in Escherichia coli, Bacillus methanolicus MGA3 Bacillus methanolicus
3.1.3.37 expressed in Corynebacterium glutamicum and Escherichia coli BL21(DE3) cells Bacillus methanolicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 ADP 33% inhibition; 38% inhibition Bacillus methanolicus
3.1.3.11 ATP 50% inhibition; 56% inhibition Bacillus methanolicus
3.1.3.11 D-fructose 1,6-bisphosphate
-
 Bacillus methanolicus
3.1.3.11 EDTA at an equimolar concentration to the bivalent metal ions, almost complete loss of activity; at an equimolar concentration to the bivalent metal ions, almost complete loss of activity Bacillus methanolicus
3.1.3.11 Li+ 1 mM, 50% residual activity; 1 mM, 50% residual activity Bacillus methanolicus
3.1.3.11 additional information not inhibitory: AMP, phosphoenolpyruvate, D-fructose 1-phosphate, D-fructose 6-phosphate, and D-fructose 2,6-bisphosphate up to 5 mM; not inhibitory: AMP, phosphoenolpyruvate, D-fructose 1-phosphate, D-fructose 6-phosphate, and D-fructose 2,6-bisphosphate up to 5 mM Bacillus methanolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.014
-
 D-fructose 1,6-bisphosphate pH 7.7, 50° Bacillus methanolicus
3.1.3.11 0.44
-
 D-fructose 1,6-bisphosphate pH 7.7, 50° Bacillus methanolicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 KCl 100 mM, 20% increase in activity Bacillus methanolicus
3.1.3.11 Mn2+ activation Bacillus methanolicus
3.1.3.11 additional information no significant activation: Co2+, Ni2+, Cu2+, Zn2+, Fe2+, and Ca2+ Bacillus methanolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.11 33132
-
 4 * 33132, calculated Bacillus methanolicus
3.1.3.11 35145
-
 4 * 35145, calculated Bacillus methanolicus
3.1.3.11 135000
-
 gel filtration Bacillus methanolicus
3.1.3.11 142000
-
 gel filtration Bacillus methanolicus
3.1.3.37 33000
-
 4 * 33000, SDS-PAGE Bacillus methanolicus
3.1.3.37 132000
-
 gel filtration Bacillus methanolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.37 sedoheptulose 1,7-bisphosphate + H2O Bacillus methanolicus
-
 sedoheptulose 7-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Bacillus methanolicus I3DTM3 chromosome-encoded isoform; i.e. MGA3
-
3.1.3.11 Bacillus methanolicus Q6TV42 plasmid-encoded isoform
-
3.1.3.11 Bacillus methanolicus ATCC 53907 I3DTM3 chromosome-encoded isoform; i.e. MGA3
-
3.1.3.11 Bacillus methanolicus ATCC 53907 Q6TV42 plasmid-encoded isoform
-
3.1.3.37 Bacillus methanolicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.37
-
 Bacillus methanolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Bacillus methanolicus D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Bacillus methanolicus ATCC 53907 D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 additional information the chromosome-encoded isoform is the major fructose 1,6-bisphosphatase of Bacillus methanolicus Bacillus methanolicus ?
-
 ?
3.1.3.11 additional information the chromosome-encoded isoform is the major fructose 1,6-bisphosphatase of Bacillus methanolicus Bacillus methanolicus ATCC 53907 ?
-
 ?
3.1.3.37 sedoheptulose 1,7-bisphosphate + H2O
-
 Bacillus methanolicus sedoheptulose 7-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.11 tetramer 4 * 33132, calculated Bacillus methanolicus
3.1.3.11 tetramer 4 * 35145, calculated Bacillus methanolicus
3.1.3.37 homotetramer 4 * 33000, SDS-PAGE Bacillus methanolicus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.11 GlpXp
-
 Bacillus methanolicus
3.1.3.37 GlpXp bifunctional enzyme with sedoheptulose 1,7-bisphosphatase and fructose 1,6-bisphosphatase activities Bacillus methanolicus
3.1.3.37 SBPase
-
 Bacillus methanolicus
3.1.3.37 sedoheptulose 1,7-bisphosphatase
-
 Bacillus methanolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.37 55
-
-
 Bacillus methanolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.11 60
-
 stable up to Bacillus methanolicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.11 1.2
-
 D-fructose 1,6-bisphosphate pH 7.7, 50° Bacillus methanolicus
3.1.3.11 3.9
-
 D-fructose 1,6-bisphosphate pH 7.7, 50° Bacillus methanolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.11 8.5 9
-
 Bacillus methanolicus
3.1.3.37 8.5 9
-
 Bacillus methanolicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.11 7 10
-
 Bacillus methanolicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.11 3.5
-
 D-fructose 1,6-bisphosphate pH 7.7, 50°C Bacillus methanolicus

General Information

EC Number General Information Comment Organism
3.1.3.11 physiological function a fructose bisphosphatase-negative Corynebacterium glutamicum mutant can be phenotypically complemented with both the chromosome-encoded and the plasmid-encoded isoforms from Bacillus methanolicus Bacillus methanolicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.11 8.8
-
 D-fructose 1,6-bisphosphate pH 7.7, 50° Bacillus methanolicus
3.1.3.11 86.3
-
 D-fructose 1,6-bisphosphate pH 7.7, 50° Bacillus methanolicus