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Literature summary extracted from

  • Smeulders, M.J.; Pol, A.; Venselaar, H.; Barends, T.R.; Hermans, J.; Jetten, M.S.; Op den Camp, H.J.
    Bacterial CS2 hydrolases from Acidithiobacillus thiooxidans strains are homologous to the archaeal catenane CS2 hydrolase (2013), J. Bacteriol., 195, 4046-4056.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.1.5 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis Acidithiobacillus thiooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.1.5 0.014
-
 carbonyl sulfide pH 7.0, 30°C Acidithiobacillus thiooxidans
3.13.1.5 0.046
-
 carbon disulfide pH 7.0, 30°C Acidithiobacillus thiooxidans
3.13.1.5 0.074
-
 carbonyl sulfide pH 7.0, 30°C Acidithiobacillus thiooxidans
3.13.1.5 0.093
-
 carbon disulfide pH 7.0, 30°C Acidithiobacillus thiooxidans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.1.5 carbon disulfide + 2 H2O Acidithiobacillus thiooxidans
-
 CO2 + 2 hydrogen sulfide
-
 ?
3.13.1.5 carbon disulfide + 2 H2O Acidithiobacillus thiooxidans S1p
-
 CO2 + 2 hydrogen sulfide
-
 ?
3.13.1.5 carbon disulfide + 2 H2O Acidithiobacillus thiooxidans Sts 4-3
-
 CO2 + 2 hydrogen sulfide
-
 ?
3.13.1.5 carbon disulfide + 2 H2O Acidithiobacillus thiooxidans 2Bp
-
 CO2 + 2 hydrogen sulfide
-
 ?
3.13.1.5 carbon disulfide + 2 H2O Acidithiobacillus thiooxidans BBW1
-
 CO2 + 2 hydrogen sulfide
-
 ?
3.13.1.5 carbon disulfide + 2 H2O Acidithiobacillus thiooxidans G8
-
 CO2 + 2 hydrogen sulfide
-
 ?
3.13.1.5 carbonyl sulfide + H2O Acidithiobacillus thiooxidans
-
 CO2 + H2S
-
 ?
3.13.1.5 carbonyl sulfide + H2O Acidithiobacillus thiooxidans S1p
-
 CO2 + H2S
-
 ?
3.13.1.5 carbonyl sulfide + H2O Acidithiobacillus thiooxidans G8
-
 CO2 + H2S
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.13.1.5 Acidithiobacillus thiooxidans
-
 isolated from mixed hot spring samples
-
3.13.1.5 Acidithiobacillus thiooxidans
-
 isolated from Solfatara, Naples
-
3.13.1.5 Acidithiobacillus thiooxidans
-
 sample isolated from CS2-removing biotrickling filters
-
3.13.1.5 Acidithiobacillus thiooxidans S5FT07 iolated from Solfarata, Rome
-
3.13.1.5 Acidithiobacillus thiooxidans S5FU55 sample isolated from CS2-removing biotrickling filters
-
3.13.1.5 Acidithiobacillus thiooxidans 2Bp
-
 isolated from mixed hot spring samples
-
3.13.1.5 Acidithiobacillus thiooxidans BBW1
-
 sample isolated from CS2-removing biotrickling filters
-
3.13.1.5 Acidithiobacillus thiooxidans G8 S5FU55 sample isolated from CS2-removing biotrickling filters
-
3.13.1.5 Acidithiobacillus thiooxidans S1p S5FT07 iolated from Solfarata, Rome
-
3.13.1.5 Acidithiobacillus thiooxidans Sts 4-3
-
 isolated from Solfatara, Naples
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.1.5 native enzyme by ammonium sulfate precipitation, followed by hydrophobic interaction chromatography, and anion exchange chromatography Acidithiobacillus thiooxidans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.13.1.5 culture condition:carbon disulfide-grown cell CS2 as sole energy source Acidithiobacillus thiooxidans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.1.5 carbon disulfide + 2 H2O
-
 Acidithiobacillus thiooxidans CO2 + 2 hydrogen sulfide
-
 ?
3.13.1.5 carbon disulfide + 2 H2O
-
 Acidithiobacillus thiooxidans S1p CO2 + 2 hydrogen sulfide
-
 ?
3.13.1.5 carbon disulfide + 2 H2O
-
 Acidithiobacillus thiooxidans Sts 4-3 CO2 + 2 hydrogen sulfide
-
 ?
3.13.1.5 carbon disulfide + 2 H2O
-
 Acidithiobacillus thiooxidans 2Bp CO2 + 2 hydrogen sulfide
-
 ?
3.13.1.5 carbon disulfide + 2 H2O
-
 Acidithiobacillus thiooxidans BBW1 CO2 + 2 hydrogen sulfide
-
 ?
3.13.1.5 carbon disulfide + 2 H2O
-
 Acidithiobacillus thiooxidans G8 CO2 + 2 hydrogen sulfide
-
 ?
3.13.1.5 carbonyl sulfide + H2O
-
 Acidithiobacillus thiooxidans CO2 + H2S
-
 ?
3.13.1.5 carbonyl sulfide + H2O
-
 Acidithiobacillus thiooxidans S1p CO2 + H2S
-
 ?
3.13.1.5 carbonyl sulfide + H2O
-
 Acidithiobacillus thiooxidans G8 CO2 + H2S
-
 ?
3.13.1.5 additional information enzyme substrate specificity, overview Acidithiobacillus thiooxidans ?
-
 ?
3.13.1.5 additional information enzyme substrate specificity, overview Acidithiobacillus thiooxidans S1p ?
-
 ?
3.13.1.5 additional information enzyme substrate specificity, overview Acidithiobacillus thiooxidans G8 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.13.1.5 hexadecamer the enzyme exhibits the unique hexadecameric catenane structure, native PAGE and analytical ultracentrifugation Acidithiobacillus thiooxidans
3.13.1.5 octamer the enzyme does not exhibit the unique hexadecameric catenane structure, S1p CS2 hydrolase forms only the single octameric ring, native PAGE and analytical ultracentrifugation Acidithiobacillus thiooxidans

Synonyms

EC Number Synonyms Comment Organism
3.13.1.5 carbon disulfide hydrolase
-
 Acidithiobacillus thiooxidans
3.13.1.5 CS2 hydrolase
-
 Acidithiobacillus thiooxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.13.1.5 30
-
 assay at Acidithiobacillus thiooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.13.1.5 7
-
 assay at Acidithiobacillus thiooxidans

General Information

EC Number General Information Comment Organism
3.13.1.5 additional information structure modeling, overview Acidithiobacillus thiooxidans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.13.1.5 8400
-
 carbon disulfide pH 7.0, 30°C Acidithiobacillus thiooxidans
3.13.1.5 11000
-
 carbonyl sulfide pH 7.0, 30°C Acidithiobacillus thiooxidans
3.13.1.5 64000
-
 carbon disulfide pH 7.0, 30°C Acidithiobacillus thiooxidans
3.13.1.5 160000
-
 carbonyl sulfide pH 7.0, 30°C Acidithiobacillus thiooxidans