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Literature summary extracted from
- cDNA cloning, expression, and enzymatic activity of a novel endogenous cellulase from the beetle Batocera horsfieldi (2013), Gene, 514, 62-68.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.4 | expression in baculovirus-infected insect BmN cells and Bombyx mori larvae | Batocera horsfieldi |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 28000 | - |
x * 28000, calculated, x * 28000, and x * 33000, SDS-PAGE of unglycosylated and glycosylated protein, respectively | Batocera horsfieldi |
| 3.2.1.4 | 33000 | - |
x * 28000, calculated, x * 28000, and x * 33000, SDS-PAGE of unglycosylated and glycosylated protein, respectively | Batocera horsfieldi |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.4 | Batocera horsfieldi | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | glycoprotein | protein sequence has three putative N-glycosylation sites at residues 56-58, 99-101, and 237-239, respectively. Gylcosylated protein shows a molecular mass of 33 kDa compared with 28 kDa for ungycosylated form | Batocera horsfieldi |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 928 | - |
pH 6.0, 50°C | Batocera horsfieldi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.4 | carboxymethyl cellulose + H2O | - |
Batocera horsfieldi | ? | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | ? | x * 28000, calculated, x * 28000, and x * 33000, SDS-PAGE of unglycosylated and glycosylated protein, respectively | Batocera horsfieldi |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | EGase II | - |
Batocera horsfieldi |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 50 | - |
- |
Batocera horsfieldi |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 6 | - |
- |
Batocera horsfieldi |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.4 | Batocera horsfieldi | calculated | - |
7.6 |
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Release 2023.1 (January 2023)
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