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Literature summary extracted from

  • de Assis, C.; Linhares, A.; Oliveira, V.; Fran็a, R.; Santos, J.; Marcuschi, M.; Maciel Carvalho, E.; Bezerra, R.; Carvalho Jr., L.
    Characterization of catalytic efficiency parameters of brain cholinesterases in tropical fish (2014), Fish Physiol. Biochem., 40, 1659-1668.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 BW284c51 i.e. 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide Arapaima gigas
3.1.1.7 BW284c51 i.e. 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide Colossoma macropomum
3.1.1.7 BW284c51 i.e. 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide Oreochromis niloticus
3.1.1.7 BW284c51 i.e. 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide Rachycentron canadum
3.1.1.7 eserine
-
 Arapaima gigas
3.1.1.7 eserine
-
 Colossoma macropomum
3.1.1.7 eserine
-
 Oreochromis niloticus
3.1.1.7 eserine
-
 Rachycentron canadum
3.1.1.7 additional information tetraisopropyl diphosphoramide does not reach 50% inhibition in the concentration range of the assays (0.001-10 mM) Colossoma macropomum
3.1.1.7 additional information tetraisopropyl diphosphoramide does not reach 50% inhibition in the concentration range of the assays (0.001-10 mM) Oreochromis niloticus
3.1.1.7 additional information tetraisopropyl diphosphoramide does not reach 50% inhibition in the concentration range of the assays (0.001-10 mM) Rachycentron canadum
3.1.1.7 neostigmine bromide
-
 Arapaima gigas
3.1.1.7 neostigmine bromide
-
 Colossoma macropomum
3.1.1.7 neostigmine bromide
-
 Oreochromis niloticus
3.1.1.7 neostigmine bromide
-
 Rachycentron canadum
3.1.1.7 tetraisopropyl diphosphoramide
-
 Arapaima gigas

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.7 0.39
-
 acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus
3.1.1.7 0.42
-
 acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas
3.1.1.7 0.43
-
 acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum
3.1.1.7 0.43
-
 acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum
3.1.1.7 0.63
-
 propionylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum
3.1.1.7 1.02
-
 propionylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas
3.1.1.7 1.61
-
 butyrylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum
3.1.1.7 2.24
-
 propionylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum
3.1.1.7 2.9
-
 propionylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus
3.1.1.7 4.23
-
 butyrylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum
3.1.1.7 5.14
-
 butyrylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.7 244000
-
 x * 244000, SDS-PAGE Colossoma macropomum
3.1.1.7 244000
-
 x * 244000, SDS-PAGE Rachycentron canadum
3.1.1.7 252000
-
 x * 252000, SDS-PAGE Oreochromis niloticus
3.1.1.7 252000
-
 x * 252000, SDS-PAGE Colossoma macropomum
3.1.1.7 287000
-
 x * 287000, SDS-PAGE Colossoma macropomum
3.1.1.7 299000
-
 x * 299000, SDS-PAGE Colossoma macropomum
3.1.1.7 299000
-
 x * 299000, SDS-PAGE Arapaima gigas

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Arapaima gigas
-
-
-
3.1.1.7 Colossoma macropomum
-
-
-
3.1.1.7 Oreochromis niloticus
-
-
-
3.1.1.7 Rachycentron canadum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.7 brain
-
 Oreochromis niloticus
-
3.1.1.7 brain
-
 Colossoma macropomum
-
3.1.1.7 brain
-
 Rachycentron canadum
-
3.1.1.7 brain
-
 Arapaima gigas
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.7 acetylthiocholine + H2O most efficiently hydrolyzed substrate Oreochromis niloticus acetate + thiocholine
-
 ?
3.1.1.7 acetylthiocholine + H2O most efficiently hydrolyzed substrate Colossoma macropomum acetate + thiocholine
-
 ?
3.1.1.7 acetylthiocholine + H2O most efficiently hydrolyzed substrate Rachycentron canadum acetate + thiocholine
-
 ?
3.1.1.7 acetylthiocholine + H2O most efficiently hydrolyzed substrate Arapaima gigas acetate + thiocholine
-
 ?
3.1.1.7 butyrylthiocholine + H2O
-
 Oreochromis niloticus butyrate + thiocholine
-
 ?
3.1.1.7 butyrylthiocholine + H2O
-
 Colossoma macropomum butyrate + thiocholine
-
 ?
3.1.1.7 butyrylthiocholine + H2O
-
 Rachycentron canadum butyrate + thiocholine
-
 ?
3.1.1.7 butyrylthiocholine + H2O
-
 Arapaima gigas butyrate + thiocholine
-
 ?
3.1.1.7 propionylthiocholine + H2O
-
 Oreochromis niloticus propanoate + thiocholine
-
 ?
3.1.1.7 propionylthiocholine + H2O
-
 Colossoma macropomum propanoate + thiocholine
-
 ?
3.1.1.7 propionylthiocholine + H2O
-
 Rachycentron canadum propanoate + thiocholine
-
 ?
3.1.1.7 propionylthiocholine + H2O
-
 Arapaima gigas propanoate + thiocholine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.7 ? x * 252000, SDS-PAGE Oreochromis niloticus
3.1.1.7 ? x * 252000, SDS-PAGE Colossoma macropomum
3.1.1.7 ? x * 244000, SDS-PAGE Colossoma macropomum
3.1.1.7 ? x * 244000, SDS-PAGE Rachycentron canadum
3.1.1.7 ? x * 287000, SDS-PAGE Colossoma macropomum
3.1.1.7 ? x * 299000, SDS-PAGE Colossoma macropomum
3.1.1.7 ? x * 299000, SDS-PAGE Arapaima gigas

Synonyms

EC Number Synonyms Comment Organism
3.1.1.7 AChE
-
 Oreochromis niloticus
3.1.1.7 AChE
-
 Colossoma macropomum
3.1.1.7 AChE
-
 Rachycentron canadum
3.1.1.7 AChE
-
 Arapaima gigas

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.7 1.04
-
 acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas
3.1.1.7 1.29
-
 acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum
3.1.1.7 1.55
-
 acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus
3.1.1.7 1.84
-
 acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.7 0.0000009
-
 neostigmine bromide in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus
3.1.1.7 0.000001
-
 neostigmine bromide in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum
3.1.1.7 0.000002
-
 neostigmine bromide in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum
3.1.1.7 0.0000021
-
 eserine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas
3.1.1.7 0.0000025
-
 eserine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum
3.1.1.7 0.000004
-
 neostigmine bromide in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas
3.1.1.7 0.0000045
-
 eserine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus
3.1.1.7 0.0000055
-
 eserine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum
3.1.1.7 0.00002
-
 BW284c51 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus
3.1.1.7 0.000045
-
 BW284c51 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum
3.1.1.7 0.00005
-
 BW284c51 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum
3.1.1.7 0.00219
-
 BW284c51 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas
3.1.1.7 0.00219
-
 tetraisopropyl diphosphoramide in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.7 0.00015
-
 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum neostigmine bromide
3.1.1.7 0.00016
-
 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus neostigmine bromide
3.1.1.7 0.00017
-
 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum neostigmine bromide
3.1.1.7 0.00031
-
 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas eserine
3.1.1.7 0.000371
-
 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum eserine
3.1.1.7 0.00049
-
 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum eserine
3.1.1.7 0.00059
-
 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas neostigmine bromide
3.1.1.7 0.00076
-
 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus eserine
3.1.1.7 0.00396
-
 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum BW284c51
3.1.1.7 0.00412
-
 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus BW284c51
3.1.1.7 0.00752
-
 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum BW284c51
3.1.1.7 0.3184
-
 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas BW284c51
3.1.1.7 0.3184
-
 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas tetraisopropyl diphosphoramide

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.7 23000
-
 acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum
3.1.1.7 24700
-
 acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas
3.1.1.7 39700
-
 acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus
3.1.1.7 42000
-
 acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum