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Literature summary extracted from

  • Deng, X.; Petitjean, M.; Teste, M.; Kooli, W.; Tranier, S.; Francois, J.; Parrou, J.
    Similarities and differences in the biochemical and enzymological properties of the four isomaltases from Saccharomyces cerevisiae (2014), FEBS Open Bio, 4, 200-212.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.10 L240P similar to wild-type Saccharomyces cerevisiae
3.2.1.10 R279Q displays substrate specificities and specific activities close to those of isoform Ima2 Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.10 4-nitrophenyl alpha-D-glucopyranoside
-
 Saccharomyces cerevisiae
3.2.1.10 isomaltose
-
 Saccharomyces cerevisiae
3.2.1.10 maltose competitive; competitive; mixed Saccharomyces cerevisiae
3.2.1.10 methyl alpha-D-glucopyranoside
-
 Saccharomyces cerevisiae
3.2.1.10 palatinose
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.10 0.35
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 0.48
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 0.58
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 0.89
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 7
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 11
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 12
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 13
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 15
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 17
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 18
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 27
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 28
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 37
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 72
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 83
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 116
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 128
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 144
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 147
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 191
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.10 Saccharomyces cerevisiae P0CW40 isoform Ima3
-
3.2.1.10 Saccharomyces cerevisiae P40884 isoform Ima5
-
3.2.1.10 Saccharomyces cerevisiae P53051 isoform Ima1
-
3.2.1.10 Saccharomyces cerevisiae Q08295 isoform Ima2
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.10 1.2
-
 substrate sucrose, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 3.9
-
 substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 5
-
 substrate palatinose, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 5.8
-
 substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 7.2
-
 substrate isomaltose, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 7.6
-
 substrate isomaltriose, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 7.7
-
 substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 7.9
-
 substrate isomaltriose, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 8.6
-
 substrate isomaltriose, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 10
-
 substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 10.2
-
 substrate sucrose, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 11
-
 substrate methyl alpha-D-glucopyranoside, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 18.6
-
 substrate sucrose, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 19.7
-
 substrate sucrose, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 39
-
 substrate palatinose, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 52
-
 substrate methyl alpha-D-glucopyranoside, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 52
-
 substrate palatinose, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 54
-
 substrate isomaltose, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 55
-
 substrate methyl alpha-D-glucopyranoside, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 60
-
 substrate isomaltose, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 65
-
 substrate isomaltose, pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 74
-
 substrate palatinose, pH 7.0, 30°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.10 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Saccharomyces cerevisiae 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.10 isomaltose + H2O
-
 Saccharomyces cerevisiae 2 D-glucose
-
 ?
3.2.1.10 isomaltriose + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.2.1.10 methyl alpha-D-glucopyranoside + H2O
-
 Saccharomyces cerevisiae methanol + alpha-D-glucopyranose
-
 ?
3.2.1.10 additional information isoforms Ima1, Ima2, Ima3 and Ima5 exhibit a preference for the alpha-(1,6) disaccharides isomaltose and palatinose, with Michaelis-Menten kinetics and inhibition at high substrates concentration. They are also able to hydrolyze trisaccharides bearing an alpha-(1,6) linkage, but also alpha-(1,2), alpha-(1,3) and alpha-(1,5) disaccharides including sucrose, highlighting their substrate ambiguity. No substrate: melibiose Saccharomyces cerevisiae ?
-
 ?
3.2.1.10 additional information isoforms Ima1, Ima2, Ima3 and Ima5 exhibit a preference for the alpha-(1,6) disaccharides isomaltose and palatinose, with Michaelis–Menten kinetics and inhibition at high substrates concentration. They are also able to hydrolyze trisaccharides bearing an alpha-(1,6) linkage, but also alpha-(1,2), alpha-(1,3) and alpha-(1,5) disaccharides including sucrose, highlighting their substrate ambiguity. No substrate: melibiose Saccharomyces cerevisiae ?
-
 ?
3.2.1.10 palatinose + H2O
-
 Saccharomyces cerevisiae D-glucose + D-fructose
-
 ?
3.2.1.10 sucrose + H2O
-
 Saccharomyces cerevisiae D-glucose + D-fructose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.10 Ima2
-
 Saccharomyces cerevisiae
3.2.1.10 Ima3
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.10 36
-
-
 Saccharomyces cerevisiae
3.2.1.10 43
-
-
 Saccharomyces cerevisiae
3.2.1.10 46
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.10 29.9
-
 melting temperature, pH 8 Saccharomyces cerevisiae
3.2.1.10 33.2
-
 melting temperature, pH 8 Saccharomyces cerevisiae
3.2.1.10 35.7
-
 melting temperature, pH 8 Saccharomyces cerevisiae
3.2.1.10 36.3
-
 melting temperature, pH 7 Saccharomyces cerevisiae
3.2.1.10 36.6
-
 melting temperature, pH 6 Saccharomyces cerevisiae
3.2.1.10 37
-
 half-life 2.9 min Saccharomyces cerevisiae
3.2.1.10 37
-
 half-life 23.2 min Saccharomyces cerevisiae
3.2.1.10 37
-
 half-life 26.1 min Saccharomyces cerevisiae
3.2.1.10 37
-
 half-life 27.4 min Saccharomyces cerevisiae
3.2.1.10 38.8
-
 melting temperature, pH 7 Saccharomyces cerevisiae
3.2.1.10 40
-
 half-life 7.6 min Saccharomyces cerevisiae
3.2.1.10 40
-
 half-life 17.6 min Saccharomyces cerevisiae
3.2.1.10 40
-
 half-life 0.8 min Saccharomyces cerevisiae
3.2.1.10 40
-
 half-life 18.5 min Saccharomyces cerevisiae
3.2.1.10 41.6
-
 melting temperature, pH 8 Saccharomyces cerevisiae
3.2.1.10 46.2
-
 melting temperature, pH 7 Saccharomyces cerevisiae
3.2.1.10 46.6
-
 melting temperature, pH 7 Saccharomyces cerevisiae
3.2.1.10 47
-
 melting temperature, pH 6 Saccharomyces cerevisiae
3.2.1.10 55.1
-
 melting temperature, pH 6 Saccharomyces cerevisiae
3.2.1.10 55.5
-
 melting temperature, pH 6 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.10 3.8
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 6
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 7.1
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 8.7
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 14
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 14
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 15
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 15
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 18
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 21
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 25
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 51
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 55
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 56
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 78
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 84
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 88
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 97
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 101
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 103
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 104
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.10 7
-
-
 Saccharomyces cerevisiae
3.2.1.10 7.6
-
-
 Saccharomyces cerevisiae
3.2.1.10 8
-
-
 Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.10 2 5 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 9.8
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 12
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 188
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 246
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 248
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 574
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 600
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 686
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 690
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.10 0.02
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 0.16
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 0.21
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 0.22
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 0.22
-
 isomaltriose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 0.35
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 0.38
-
 sucrose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 0.41
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 0.8
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 0.9
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 3.1
-
 methyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 4.3
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 5
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 5.6
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 5.7
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 8
-
 isomaltose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 9.4
-
 palatinose pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 15
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 16
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 20
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae
3.2.1.10 28
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C Saccharomyces cerevisiae