BRENDA - Enzyme Database

Crystal structure of an efficacious gonococcal adherence inhibitor: An enolase from Lactobacillus gasseri

Raghunathan, K.; Harris, P.T.; Spurbeck, R.R.; Arvidson, C.G.; Arvidson, D.N.; FEBS Lett. 588, 2212-2216 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli
Lactobacillus gasseri
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.2.1.11
hanging drop vapor diffusion method, using 20% (w/v) PEG 550, 100 mM bicine pH 9.0, 100 mM sodium chloride
Lactobacillus gasseri
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
each monomer is binding a single Mg2+ cofactor
Lactobacillus gasseri
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Lactobacillus gasseri
-
phosphoenolpyruvate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Lactobacillus gasseri
Q042F4
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
nickel affinity column chromatography
Lactobacillus gasseri
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
729739
Lactobacillus gasseri
phosphoenolpyruvate + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli
Lactobacillus gasseri
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.2.1.11
hanging drop vapor diffusion method, using 20% (w/v) PEG 550, 100 mM bicine pH 9.0, 100 mM sodium chloride
Lactobacillus gasseri
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
each monomer is binding a single Mg2+ cofactor
Lactobacillus gasseri
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Lactobacillus gasseri
-
phosphoenolpyruvate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
nickel affinity column chromatography
Lactobacillus gasseri
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
729739
Lactobacillus gasseri
phosphoenolpyruvate + H2O
-
-
-
?