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Literature summary extracted from

  • Haagen, Y.; Unsoeld, I.; Westrich, L.; Gust, B.; Richard, S.B.; Noel, J.P.; Heide, L.
    A soluble, magnesium-independent prenyltransferase catalyzes reverse and regular C-prenylations and O-prenylations of aromatic substrates (2007), FEBS Lett., 581, 2889-2893.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.123 expressed in Escherichia coli BL21(DE3)pLysS cells Streptomyces virginiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.123 additional information addition of EDTA (10 mM) to an assay not containing any divalent cations does not affect the enzyme activity Streptomyces virginiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.123 0.0021
-
 geranyl diphosphate with flaviolin as cosubstrate, in 100 mM Na-TAPS (pH 8.5), at 30°C Streptomyces virginiae
2.5.1.123 0.0027
-
 geranyl diphosphate with 1,3-dihydroxynaphthalene as cosubstrate, in 100 mM Na-TAPS (pH 8.5), at 30°C Streptomyces virginiae
2.5.1.123 0.021
-
 flaviolin in 100 mM Na-TAPS (pH 8.5), at 30°C Streptomyces virginiae
2.5.1.123 1.9
-
 1,3-dihydroxynaphthalene in 100 mM Na-TAPS (pH 8.5), at 30°C Streptomyces virginiae
2.5.1.123 5.6
-
 4-hydroxybenzoate in 100 mM Na-TAPS (pH 8.5), at 30°C Streptomyces virginiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.123 additional information the enzyme activity is independent of the presence of Mg2+ or other divalent metal ions Streptomyces virginiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.123 32700
-
 1 * 32700, calculated from amino acid sequence Streptomyces virginiae
2.5.1.123 33200
-
 1 * 33200, SDS-PAGE Streptomyces virginiae
2.5.1.123 39100
-
 gel filtration Streptomyces virginiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.123 geranyl diphosphate + flaviolin Streptomyces virginiae flaviolin is 2,5,7-trihydroxy-1,4-naphthoquinone 3-linalylflaviolin + diphosphate
-
 ?
2.5.1.123 geranyl diphosphate + flaviolin Streptomyces virginiae DSM 1042 flaviolin is 2,5,7-trihydroxy-1,4-naphthoquinone 3-linalylflaviolin + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.123 Streptomyces virginiae
-
-
-
2.5.1.123 Streptomyces virginiae DSM 1042
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.123 Ni-NTA-agarose resin column chromatography, benzamidine-Sepharose column chromatography, and Superdex 200 gel filtration Streptomyces virginiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.123 geranyl diphosphate + 1,3-dihydroxynaphthalene
-
 Streptomyces virginiae 3-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]naphthalen-1-ol + 4-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]naphthalen-2-ol
-
 ?
2.5.1.123 geranyl diphosphate + 1,3-dihydroxynaphthalene
-
 Streptomyces virginiae DSM 1042 3-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]naphthalen-1-ol + 4-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]naphthalen-2-ol
-
 ?
2.5.1.123 geranyl diphosphate + 4-hydroxybenzoate
-
 Streptomyces virginiae 4-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]benzoic acid
-
 ?
2.5.1.123 geranyl diphosphate + 4-hydroxybenzoate
-
 Streptomyces virginiae DSM 1042 4-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]benzoic acid
-
 ?
2.5.1.123 geranyl diphosphate + flaviolin flaviolin is 2,5,7-trihydroxy-1,4-naphthoquinone Streptomyces virginiae 3-linalylflaviolin + diphosphate
-
 ?
2.5.1.123 geranyl diphosphate + flaviolin flaviolin is 2,5,7-trihydroxy-1,4-naphthoquinone Streptomyces virginiae DSM 1042 3-linalylflaviolin + diphosphate
-
 ?
2.5.1.123 additional information does not accept 1,6-dihydroxynaphthalene and 4-hydroxyphenylpyruvate as substrate Streptomyces virginiae ?
-
 ?
2.5.1.123 additional information does not accept 1,6-dihydroxynaphthalene and 4-hydroxyphenylpyruvate as substrate Streptomyces virginiae DSM 1042 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.123 monomer 1 * 33200, SDS-PAGE Streptomyces virginiae
2.5.1.123 monomer 1 * 32700, calculated from amino acid sequence Streptomyces virginiae

Synonyms

EC Number Synonyms Comment Organism
2.5.1.123 Fnq26
-
 Streptomyces virginiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.123 0.0002
-
 flaviolin in 100 mM Na-TAPS (pH 8.5), at 30°C Streptomyces virginiae
2.5.1.123 0.002
-
 4-hydroxybenzoate in 100 mM Na-TAPS (pH 8.5), at 30°C Streptomyces virginiae
2.5.1.123 0.003
-
 1,3-dihydroxynaphthalene in 100 mM Na-TAPS (pH 8.5), at 30°C Streptomyces virginiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.123 8.5
-
-
 Streptomyces virginiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.123 7.5 9.2 half-maximal values at pH 7.5 and 9.2 Streptomyces virginiae

General Information

EC Number General Information Comment Organism
2.5.1.123 metabolism the enzyme is involved in FNQ I biosynthesis Streptomyces virginiae