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Literature summary extracted from

  • Rawat, R.; Tewari, L.
    Purification and characterization of an acidothermophilic cellulase enzyme produced by Bacillus subtilis strain LFS3 (2012), Extremophiles, 16, 637-644.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Fe3+ 5 mM, 5% residual activity Bacillus subtilis
3.2.1.4 Hg2+ 5 mM, 10% residual activity Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Na+ 5 mM, 130% of initial activity Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 185000
-
 PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Bacillus subtilis
-
-
-
3.2.1.4 Bacillus subtilis LFS3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 14.4
-
 pH 4.0, 60°C Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Bacillus subtilis LFS3 ?
-
 ?
3.2.1.4 filter paper + H2O 8.4% of the activity with carboxymethyl cellulose Bacillus subtilis ?
-
 ?
3.2.1.4 filter paper + H2O 8.4% of the activity with carboxymethyl cellulose Bacillus subtilis LFS3 ?
-
 ?
3.2.1.4 additional information no substrates: 4-nitrophenyl beta-D-glucopyranoside, cellulose Bacillus subtilis ?
-
 ?
3.2.1.4 additional information no substrates: 4-nitrophenyl beta-D-glucopyranoside, cellulose Bacillus subtilis LFS3 ?
-
 ?
3.2.1.4 xylan + H2O 20.3% of the activity with carboxymethyl cellulose Bacillus subtilis ?
-
 ?
3.2.1.4 xylan + H2O 20.3% of the activity with carboxymethyl cellulose Bacillus subtilis LFS3 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 60
-
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 40
-
 80% of maximum activity Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 80
-
 30 min, 29% residual activity Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 4
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 3 8 more than 75% of maximum activity Bacillus subtilis