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Literature summary extracted from

  • Heuts, D.P.; Weissenborn, M.J.; Olkhov, R.V.; Shaw, A.M.; Gummadova, J.; Levy, C.; Scrutton, N.S.
    Crystal structure of a soluble form of human CD73 with ecto-5-nucleotidase activity (2012), ChemBioChem, 13, 2384-2391.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.5 soluble form of human soluble CD73 at 2.2 A resolution, a truncated form of CD73, residues 27–549, that retains ecto-5'-nucleotidase activity. Structure reveals a conserved loop that is directly involved in the dimer-dimer interaction showing that the two subunits of the dimer are not linked by disulfide bridges Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 Cu2+ further inhibition of the residual activity in EDTA-treated enzyme Homo sapiens
3.1.3.5 EDTA 2 mM, 5% residual activity Homo sapiens
3.1.3.5 Fe2+ further inhibition of the residual activity in EDTA-treated enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.0038
-
 5'-AMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 0.0065
-
 5'-GMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 0.0071
-
 5'-IMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 0.008
-
 5'-TMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 0.0316
-
 5'-UMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 0.0564
-
 5'-CMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 0.114
-
 5'-dAMP 37°C, pH not specified in the publication Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 soluble
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Co2+ 30% recovery of initial activity with EDTA-treated enzyme Homo sapiens
3.1.3.5 additional information no effect: Mg2+ Homo sapiens
3.1.3.5 additional information divalent metal ion is required Homo sapiens
3.1.3.5 Zn2+ 60% recovery of initial activity with EDTA-treated enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 65000
-
 x * 65000, truncated form of enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Homo sapiens Q6NZX3
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.5 glycoprotein sequence contains four potential gycosylation sites, Asn53, Asn311, Asn333 and Asn403. The enzyme is decorated with a variety of oligosaccharide structures Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Homo sapiens adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O
-
 Homo sapiens cytidine + phosphate
-
 ?
3.1.3.5 5'-dAMP + H2O
-
 Homo sapiens deoxyadenosine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O
-
 Homo sapiens guanosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O
-
 Homo sapiens inosine + phosphate
-
 ?
3.1.3.5 5'-TMP + H2O
-
 Homo sapiens thymidine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O
-
 Homo sapiens uridine + phosphate
-
 ?
3.1.3.5 additional information poor substrate: beta-nicotinamide mononucleotide Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 ? x * 65000, truncated form of enzyme Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.3.5 CD73
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.5 40
-
 5'-TMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 104
-
 5'-IMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 132
-
 5'-GMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 174
-
 5'-dAMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 187
-
 5'-AMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 202
-
 5'-CMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 289
-
 5'-UMP 37°C, pH not specified in the publication Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.5 1500
-
 5'-dAMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 3600
-
 5'-CMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 5000
-
 5'-TMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 9100
-
 5'-UMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 14700
-
 5'-IMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 20300
-
 5'-GMP 37°C, pH not specified in the publication Homo sapiens
3.1.3.5 48800
-
 5'-AMP 37°C, pH not specified in the publication Homo sapiens