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Literature summary extracted from
- Novel substrates for the measurement of endo-1,4-beta-glucanase (endo-cellulase) (2014), Carbohydr. Res., 385, 9-17.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | analysis | specific and sensitive assay of endo-1,4-beta-glucanase (cellulase). The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-beta-cellotrioside in the presence of thermostable beta-glucosidase. Hydrolysis by exo-acting enzymes such as beta-glucosidase and exo-beta-glucanase is prevented by the presence of the benzylidene group on the non-reducing end D-glucosyl residue. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-beta-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free D-glucose by the beta-glucosidase in the substrate mixture. The reaction is terminated and colour developed by the addition of a weak alkaline solution. The assay procedure is simple to use, specific, accurate, robust and readily adapted to automation | Thermotoga maritima |
| 3.2.1.4 | analysis | specific and sensitive assay of endo-1,4-beta-glucanase (cellulase). The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-beta-cellotrioside in the presence of thermostable beta-glucosidase. Hydrolysis by exo-acting enzymes such as beta-glucosidase and exo-beta-glucanase is prevented by the presence of the benzylidene group on the non-reducing end D-glucosyl residue. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-beta-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free D-glucose by the beta-glucosidase in the substrate mixture. The reaction is terminated and colour developed by the addition of a weak alkaline solution. The assay procedure is simple to use, specific, accurate, robust and readily adapted to automation | Trichoderma longibrachiatum |
| 3.2.1.4 | analysis | specific and sensitive assay of endo-1,4-beta-glucanase (cellulase). The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-beta-cellotrioside in the presence of thermostable beta-glucosidase. Hydrolysis by exo-acting enzymes such as beta-glucosidase and exo-beta-glucanase is prevented by the presence of the benzylidene group on the non-reducing end D-glucosyl residue. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-beta-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free D-glucose by the beta-glucosidase in the substrate mixture. The reaction is terminated and colour developed by the addition of a weak alkaline solution. The assay procedure is simple to use, specific, accurate, robust and readily adapted to automation | Bacillus amyloliquefaciens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.4 | Bacillus amyloliquefaciens | Q8RPQ6 | - |
- |
| 3.2.1.4 | Thermotoga maritima | Q9X273 | - |
- |
| 3.2.1.4 | Thermotoga maritima DSM 3109 | Q9X273 | - |
- |
| 3.2.1.4 | Trichoderma longibrachiatum | Q12714 | - |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 27.3 | - |
substrate 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside, pH 6.0, 40°C | Thermotoga maritima |
| 3.2.1.4 | 47.6 | - |
substrate 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside, pH 4.5, 40°C | Trichoderma longibrachiatum |
| 3.2.1.4 | 52.2 | - |
substrate 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside, pH 6.0, 40°C | Bacillus amyloliquefaciens |
| 3.2.1.4 | 53 | - |
substrate carboxymethyl cellulose, pH 6.0, 40°C | Thermotoga maritima |
| 3.2.1.4 | 68 | - |
substrate carboxymethyl cellulose, pH 4.5, 40°C | Trichoderma longibrachiatum |
| 3.2.1.4 | 82 | - |
substrate carboxymethyl cellulose, pH 6.0, 40°C | Bacillus amyloliquefaciens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.4 | 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside + H2O | specific and sensitive assay of endo-1,4-beta-glucanase (cellulase). The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-beta-cellotrioside in the presence of thermostable beta-glucosidase. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-beta-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free D-glucose by the beta-glucosidase in the substrate mixture | Thermotoga maritima | 4'',6''-O-benzylidene beta-cellobioside + 2-chloro-4-nitrophenyl beta-D-glucoside | - |
? |  |
| 3.2.1.4 | 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside + H2O | specific and sensitive assay of endo-1,4-beta-glucanase (cellulase). The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-beta-cellotrioside in the presence of thermostable beta-glucosidase. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-beta-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free D-glucose by the beta-glucosidase in the substrate mixture | Trichoderma longibrachiatum | 4'',6''-O-benzylidene beta-cellobioside + 2-chloro-4-nitrophenyl beta-D-glucoside | - |
? | |
| 3.2.1.4 | 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside + H2O | specific and sensitive assay of endo-1,4-beta-glucanase (cellulase). The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-beta-cellotrioside in the presence of thermostable beta-glucosidase. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-beta-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free D-glucose by the beta-glucosidase in the substrate mixture | Bacillus amyloliquefaciens | 4'',6''-O-benzylidene beta-cellobioside + 2-chloro-4-nitrophenyl beta-D-glucoside | - |
? | |
| 3.2.1.4 | 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside + H2O | specific and sensitive assay of endo-1,4-beta-glucanase (cellulase). The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-beta-cellotrioside in the presence of thermostable beta-glucosidase. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-beta-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free D-glucose by the beta-glucosidase in the substrate mixture | Thermotoga maritima DSM 3109 | 4'',6''-O-benzylidene beta-cellobioside + 2-chloro-4-nitrophenyl beta-D-glucoside | - |
? | |
| 3.2.1.4 | carboxymethyl cellulose + H2O | - |
Thermotoga maritima | ? | - |
? | |
| 3.2.1.4 | carboxymethyl cellulose + H2O | - |
Trichoderma longibrachiatum | ? | - |
? | |
| 3.2.1.4 | carboxymethyl cellulose + H2O | - |
Bacillus amyloliquefaciens | ? | - |
? | |
| 3.2.1.4 | carboxymethyl cellulose + H2O | - |
Thermotoga maritima DSM 3109 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | Egl1 | - |
Trichoderma longibrachiatum |
| 3.2.1.4 | EngA | - |
Bacillus amyloliquefaciens |
| 3.2.1.4 | TM_1751 | - |
Thermotoga maritima |
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