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Literature summary extracted from
- Production of 10-hydroxy-12,15(Z,Z)-octadecadienoic acid from alpha-linolenic acid by permeabilized cells of recombinant Escherichia coli expressing the oleate hydratase gene of Stenotrophomonas maltophilia (2013), Biotechnol. Lett., 35, 1487-1493.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.53 | EDTA | maximum activity at 2 M | Stenotrophomonas maltophilia |  |
| 4.2.1.53 | NaCl | maximum activity at 125 mM | Stenotrophomonas maltophilia | |
| 4.2.1.53 | Tween 40 | optimum activity with 0.1% (v/v) Tween 40 | Stenotrophomonas maltophilia | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.53 | expressed in Escherichia coli ER2566 cells | Stenotrophomonas maltophilia |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.53 | Stenotrophomonas maltophilia | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.53 | alpha-linolenic acid + H2O | - |
Stenotrophomonas maltophilia | 10-hydroxy-12,15(Z,Z)-octadecadienoic acid | 82% yield after 18 h | ? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.53 | 35 | - |
- |
Stenotrophomonas maltophilia |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.53 | 7 | - |
- |
Stenotrophomonas maltophilia |
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Release 2023.1 (January 2023)
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