BRENDA - Enzyme Database show

Production of 10-hydroxy-12,15(Z,Z)-octadecadienoic acid from alpha-linolenic acid by permeabilized cells of recombinant Escherichia coli expressing the oleate hydratase gene of Stenotrophomonas maltophilia

Oh, H.J.; Shin, K.C.; Oh, D.K.; Biotechnol. Lett. 35, 1487-1493 (2013)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.53
EDTA
maximum activity at 2 M
Stenotrophomonas maltophilia
4.2.1.53
NaCl
maximum activity at 125 mM
Stenotrophomonas maltophilia
4.2.1.53
Tween 40
optimum activity with 0.1% (v/v) Tween 40
Stenotrophomonas maltophilia
Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.53
expressed in Escherichia coli ER2566 cells
Stenotrophomonas maltophilia
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.53
Stenotrophomonas maltophilia
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.53
alpha-linolenic acid
-
729466
Stenotrophomonas maltophilia
10-hydroxy-12,15(Z,Z)-octadecadienoic acid + H2O
82% yield after 18 h
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.53
35
-
-
Stenotrophomonas maltophilia
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.53
7
-
-
Stenotrophomonas maltophilia
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.53
EDTA
maximum activity at 2 M
Stenotrophomonas maltophilia
4.2.1.53
NaCl
maximum activity at 125 mM
Stenotrophomonas maltophilia
4.2.1.53
Tween 40
optimum activity with 0.1% (v/v) Tween 40
Stenotrophomonas maltophilia
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.53
expressed in Escherichia coli ER2566 cells
Stenotrophomonas maltophilia
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.53
alpha-linolenic acid
-
729466
Stenotrophomonas maltophilia
10-hydroxy-12,15(Z,Z)-octadecadienoic acid + H2O
82% yield after 18 h
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.53
35
-
-
Stenotrophomonas maltophilia
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.53
7
-
-
Stenotrophomonas maltophilia