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Literature summary extracted from

  • Kawamura, S.; Toshima, G.; Chijiiwa, Y.; Torikata, T.; Araki, T.
    Amino acid sequence of Egyptian goose egg-white lysozyme and effects of amino acid substitution on the enzymatic activity (2012), Biosci. Biotechnol. Biochem., 76, 691-698.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.17 DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, expression of enzyme mutants in Pichia pastoris, subcloning in Escherichia coli strain JM109 Alopochen aegyptiaca
3.2.1.17 sequence comparisons, phylogenetic tree Aix sponsa

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.17 F34Y site-directed mutagenesis at the substrate subsite E, the mutant shows a slightly altered product pattern compared to the wild-type enzyme Alopochen aegyptiaca
3.2.1.17 F34Y/N37G site-directed mutagenesis at the substrate subsites E/F, the mutant shows a slightly altered product pattern compared to the wild-type enzyme Alopochen aegyptiaca
3.2.1.17 F34Y/N37G/G71R site-directed mutagenesis, the mutant shows altered time course data compared to the wild-type enzyme Alopochen aegyptiaca
3.2.1.17 G71R site-directed mutagenesis, the mutant shows altered time course data compared to the wild-type enzyme Alopochen aegyptiaca
3.2.1.17 N37G site-directed mutagenesis at the substrate subsite F, the mutant shows a slightly altered product pattern compared to the wild-type enzyme Alopochen aegyptiaca

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.17 Aix sponsa Q7LZQ2
-
-
3.2.1.17 Alopochen aegyptiaca P84496
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.17 egg white
-
 Alopochen aegyptiaca
-
3.2.1.17 egg white
-
 Aix sponsa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.17 chitopentaose + H2O
-
 Alopochen aegyptiaca N-acetyl-D-glucosamine + chitobiose + chitotetraose + chitotriose amount in descending order, binding kinetics with chitotriose, overview ?
3.2.1.17 chitopentaose + H2O
-
 Aix sponsa N-acetyl-D-glucosamine + chitobiose + chitotriose + chitotetraose amount in descending order, binding kinetics with chitotriose, overview ?
3.2.1.17 lyophilized cell wall of Micrococcus luteus + H2O
-
 Alopochen aegyptiaca ?
-
 ?
3.2.1.17 lyophilized cell wall of Micrococcus luteus + H2O
-
 Aix sponsa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.17 EGL
-
 Alopochen aegyptiaca
3.2.1.17 Egyptian goose egg-white lysozyme
-
 Alopochen aegyptiaca
3.2.1.17 Egyptian goose lysozyme
-
 Alopochen aegyptiaca
3.2.1.17 WGL
-
 Aix sponsa
3.2.1.17 wood duck lysozyme
-
 Aix sponsa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.17 50
-
 assay at Alopochen aegyptiaca
3.2.1.17 50
-
 assay at Aix sponsa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.17 5
-
 assay at Alopochen aegyptiaca
3.2.1.17 5
-
 assay at Aix sponsa

General Information

EC Number General Information Comment Organism
3.2.1.17 additional information rates of cleavage of glycosidic linkages, transglycosylation, and hydration, enzyme reaction modelling, overview Alopochen aegyptiaca
3.2.1.17 additional information rates of cleavage of glycosidic linkages, transglycosylation, and hydration, enzyme reaction modelling, overview Aix sponsa