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Literature summary extracted from
- Biosynthesis, structural architecture and biotechnological potential of bacterial tannase: a molecular advancement (2014), Biores. Technol., 157, 327-340.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.20 | EDTA | - |
Lactiplantibacillus plantarum |  |
| 3.1.1.20 | SDS | 1% (v/v) SDS shows stimulatory (13.2%) effect on tannase activity | Bacillus subtilis | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.20 | 2-mercaptoethanol | - |
Bacillus subtilis | |
| 3.1.1.20 | 2-mercaptoethanol | - |
Lactiplantibacillus plantarum | |
| 3.1.1.20 | Ag+ | - |
Bacillus subtilis | |
| 3.1.1.20 | Al3+ | - |
Escherichia coli | |
| 3.1.1.20 | Ba2+ | - |
Bacillus subtilis | |
| 3.1.1.20 | Ba2+ | - |
Enterobacter cloacae | |
| 3.1.1.20 | Ca2+ | - |
Bacillus subtilis | |
| 3.1.1.20 | Co2+ | - |
Bacillus subtilis | |
| 3.1.1.20 | Co2+ | - |
Rhodococcus sp. | |
| 3.1.1.20 | Cu2+ | - |
Bacillus subtilis | |
| 3.1.1.20 | Cu2+ | - |
Enterobacter cloacae | |
| 3.1.1.20 | Cu2+ | - |
Escherichia coli | |
| 3.1.1.20 | Cu2+ | - |
Rhodococcus sp. | |
| 3.1.1.20 | DMSO | most potent inhibitor | Bacillus subtilis | |
| 3.1.1.20 | DMSO | - |
Lactiplantibacillus plantarum | |
| 3.1.1.20 | EDTA | - |
Escherichia coli | |
| 3.1.1.20 | Fe2+ | - |
Bacillus subtilis | |
| 3.1.1.20 | Fe2+ | - |
Enterobacter cloacae | |
| 3.1.1.20 | Fe3+ | - |
Bacillus subtilis | |
| 3.1.1.20 | Fe3+ | - |
Escherichia coli | |
| 3.1.1.20 | Fe3+ | - |
Rhodococcus sp. | |
| 3.1.1.20 | Hg2+ | - |
Bacillus subtilis | |
| 3.1.1.20 | Hg2+ | - |
Lactiplantibacillus plantarum | |
| 3.1.1.20 | Hg2+ | - |
Rhodococcus sp. | |
| 3.1.1.20 | K+ | - |
Bacillus subtilis | |
| 3.1.1.20 | methyl gallate | - |
Bacillus subtilis | |
| 3.1.1.20 | methyl gallate | - |
Enterobacter cloacae | |
| 3.1.1.20 | Mg2+ | - |
Lactiplantibacillus plantarum | |
| 3.1.1.20 | propyl gallate | - |
Bacillus subtilis | |
| 3.1.1.20 | propyl gallate | - |
Enterobacter cloacae | |
| 3.1.1.20 | pyrogallol | - |
Bacillus subtilis | |
| 3.1.1.20 | pyrogallol | - |
Enterobacter cloacae | |
| 3.1.1.20 | SDS | - |
Enterobacter cloacae | |
| 3.1.1.20 | Sodium azide | - |
Bacillus subtilis | |
| 3.1.1.20 | Sodium azide | - |
Enterobacter cloacae | |
| 3.1.1.20 | Sodium thioglycolate | - |
Bacillus subtilis | |
| 3.1.1.20 | Sodium thioglycolate | - |
Enterobacter cloacae | |
| 3.1.1.20 | Triton X-100 | - |
Bacillus subtilis | |
| 3.1.1.20 | Triton X-100 | - |
Enterobacter cloacae | |
| 3.1.1.20 | Triton X-100 | - |
Lactiplantibacillus plantarum | |
| 3.1.1.20 | Tween 20 | - |
Enterobacter cloacae | |
| 3.1.1.20 | Tween 60 | - |
Bacillus subtilis | |
| 3.1.1.20 | Tween 80 | - |
Bacillus subtilis | |
| 3.1.1.20 | Tween 80 | - |
Enterobacter cloacae | |
| 3.1.1.20 | Tween 80 | - |
Lactiplantibacillus plantarum | |
| 3.1.1.20 | Urea | - |
Lactiplantibacillus plantarum | |
| 3.1.1.20 | Zn2+ | - |
Bacillus subtilis | |
| 3.1.1.20 | Zn2+ | - |
Escherichia coli | |
| 3.1.1.20 | Zn2+ | - |
Lactiplantibacillus plantarum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.20 | 0.00337 | - |
Tannic acid | pH and temperature not specified in the publication | Enterobacter cloacae | |
| 3.1.1.20 | 0.445 | - |
Tannic acid | pH and temperature not specified in the publication | Bacillus subtilis | |
| 3.1.1.20 | 1.6 | - |
methyl gallate | pH and temperature not specified in the publication | Selenomonas ruminantium | |
| 3.1.1.20 | 3.7 | - |
methyl gallate | pH and temperature not specified in the publication | Enterobacter sp. | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.20 | Ca2+ | - |
Lactiplantibacillus plantarum | |
| 3.1.1.20 | K+ | - |
Lactiplantibacillus plantarum | |
| 3.1.1.20 | Mg2+ | Mg2+ is the most common requirement for optimum activity | Bacillus subtilis | |
| 3.1.1.20 | Mg2+ | Mg2+ is the most common requirement for optimum activity | Escherichia coli | |
| 3.1.1.20 | Mg2+ | Mg2+ is the most common requirement for optimum activity | Enterobacter cloacae | |
| 3.1.1.20 | Mg2+ | Mg2+ is the most common requirement for optimum activity | Lysinibacillus sphaericus | |
| 3.1.1.20 | Mg2+ | Mg2+ is the most common requirement for optimum activity | Bacillus cereus | |
| 3.1.1.20 | Mg2+ | Mg2+ is the most common requirement for optimum activity | Klebsiella pneumoniae | |
| 3.1.1.20 | Mg2+ | Mg2+ is the most common requirement for optimum activity | Selenomonas ruminantium | |
| 3.1.1.20 | Mg2+ | Mg2+ is the most common requirement for optimum activity | Rhodococcus sp. | |
| 3.1.1.20 | Mg2+ | Mg2+ is the most common requirement for optimum activity | Enterobacter sp. | |
| 3.1.1.20 | Mg2+ | Mg2+ is the most common requirement for optimum activity | Serratia ficaria | |
| 3.1.1.20 | Mn2+ | - |
Escherichia coli | |
| 3.1.1.20 | Mn2+ | - |
Enterobacter cloacae | |
| 3.1.1.20 | NH4+ | - |
Escherichia coli | |
| 3.1.1.20 | Ni2+ | - |
Escherichia coli | |
| 3.1.1.20 | Zn2+ | - |
Enterobacter cloacae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 31000 | - |
x * 31000, SDS-PAGE | Enterobacter cloacae |
| 3.1.1.20 | 46500 | - |
x * 46500, SDS-PAGE | Klebsiella pneumoniae |
| 3.1.1.20 | 50700 | - |
x * 50700, SDS-PAGE | Lactiplantibacillus plantarum |
| 3.1.1.20 | 52000 | - |
x * 52000 | Bacillus subtilis |
| 3.1.1.20 | 55000 | - |
x * 55000, SDS-PAGE | Escherichia coli |
| 3.1.1.20 | 59000 | - |
x * 59000, SDS-PAGE | Selenomonas ruminantium |
| 3.1.1.20 | 60000 | - |
x * 60000, SDS-PAGE | Rhodococcus sp. |
| 3.1.1.20 | 90000 | - |
x * 90000, SDS-PAGE | Enterobacter sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.20 | tannic acid + H2O | Lactiplantibacillus plantarum | - |
gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | Bacillus subtilis | - |
gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | Escherichia coli | - |
gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | Enterobacter cloacae | - |
gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | Lysinibacillus sphaericus | - |
gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | Bacillus cereus | - |
gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | Klebsiella pneumoniae | - |
gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | Selenomonas ruminantium | - |
gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | Rhodococcus sp. | - |
gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | Enterobacter sp. | - |
gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | Serratia ficaria | - |
gallate + D-glucose | - |
? | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.1.1.20 | Ethanol | the activity of tannase increases in presence of ethanol | Bacillus subtilis |
| 3.1.1.20 | Glycerol | the activity of tannase increases in presence of glycerol | Bacillus subtilis |
| 3.1.1.20 | isoamyl alcohol | the activity of tannase increases in presence of isoamyl alcohol | Bacillus subtilis |
| 3.1.1.20 | isopropanol | the activity of tannase increases in presence of isopropanol | Bacillus subtilis |
| 3.1.1.20 | Methanol | the activity of tannase increases in presence of methanol | Bacillus subtilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.20 | Bacillus cereus | - |
- |
- |
| 3.1.1.20 | Bacillus subtilis | - |
- |
- |
| 3.1.1.20 | Enterobacter cloacae | - |
- |
- |
| 3.1.1.20 | Enterobacter sp. | - |
- |
- |
| 3.1.1.20 | Escherichia coli | - |
- |
- |
| 3.1.1.20 | Klebsiella pneumoniae | - |
- |
- |
| 3.1.1.20 | Lactiplantibacillus plantarum | - |
- |
- |
| 3.1.1.20 | Lysinibacillus sphaericus | - |
- |
- |
| 3.1.1.20 | Rhodococcus sp. | - |
- |
- |
| 3.1.1.20 | Selenomonas ruminantium | - |
- |
- |
| 3.1.1.20 | Serratia ficaria | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.20 | ammonium sulfate precipitation followed by Sephadex G-75 gel filtration | Bacillus subtilis |
| 3.1.1.20 | ammonium sulfate precipitation, Q-Sepharose column chromatography, hydroxylapatite column chromatography, and MonoQ column chromatography | Lactiplantibacillus plantarum |
| 3.1.1.20 | DEAE-cellulose chromatography followed by Sephadex G-100 gel filtration | Enterobacter sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.20 | methyl gallate + H2O | - |
Lactiplantibacillus plantarum | gallate + methanol | - |
? | |
| 3.1.1.20 | methyl gallate + H2O | - |
Bacillus subtilis | gallate + methanol | - |
? | |
| 3.1.1.20 | methyl gallate + H2O | - |
Escherichia coli | gallate + methanol | - |
? | |
| 3.1.1.20 | methyl gallate + H2O | - |
Enterobacter cloacae | gallate + methanol | - |
? | |
| 3.1.1.20 | methyl gallate + H2O | - |
Lysinibacillus sphaericus | gallate + methanol | - |
? | |
| 3.1.1.20 | methyl gallate + H2O | - |
Bacillus cereus | gallate + methanol | - |
? | |
| 3.1.1.20 | methyl gallate + H2O | - |
Klebsiella pneumoniae | gallate + methanol | - |
? | |
| 3.1.1.20 | methyl gallate + H2O | - |
Selenomonas ruminantium | gallate + methanol | - |
? | |
| 3.1.1.20 | methyl gallate + H2O | - |
Rhodococcus sp. | gallate + methanol | - |
? | |
| 3.1.1.20 | methyl gallate + H2O | - |
Enterobacter sp. | gallate + methanol | - |
? | |
| 3.1.1.20 | methyl gallate + H2O | - |
Serratia ficaria | gallate + methanol | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Lactiplantibacillus plantarum | gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Bacillus subtilis | gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Escherichia coli | gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Enterobacter cloacae | gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Lysinibacillus sphaericus | gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Bacillus cereus | gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Klebsiella pneumoniae | gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Selenomonas ruminantium | gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Rhodococcus sp. | gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Enterobacter sp. | gallate + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Serratia ficaria | gallate + D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.20 | ? | x * 90000, SDS-PAGE | Enterobacter sp. |
| 3.1.1.20 | ? | x * 60000, SDS-PAGE | Rhodococcus sp. |
| 3.1.1.20 | ? | x * 55000, SDS-PAGE | Escherichia coli |
| 3.1.1.20 | ? | x * 59000, SDS-PAGE | Selenomonas ruminantium |
| 3.1.1.20 | ? | x * 31000, SDS-PAGE | Enterobacter cloacae |
| 3.1.1.20 | ? | x * 46500, SDS-PAGE | Klebsiella pneumoniae |
| 3.1.1.20 | ? | x * 52000 | Bacillus subtilis |
| 3.1.1.20 | ? | x * 50700, SDS-PAGE | Lactiplantibacillus plantarum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.20 | tannin acyl hydrolase | - |
Lactiplantibacillus plantarum |
| 3.1.1.20 | tannin acyl hydrolase | - |
Bacillus subtilis |
| 3.1.1.20 | tannin acyl hydrolase | - |
Escherichia coli |
| 3.1.1.20 | tannin acyl hydrolase | - |
Enterobacter cloacae |
| 3.1.1.20 | tannin acyl hydrolase | - |
Lysinibacillus sphaericus |
| 3.1.1.20 | tannin acyl hydrolase | - |
Bacillus cereus |
| 3.1.1.20 | tannin acyl hydrolase | - |
Klebsiella pneumoniae |
| 3.1.1.20 | tannin acyl hydrolase | - |
Selenomonas ruminantium |
| 3.1.1.20 | tannin acyl hydrolase | - |
Rhodococcus sp. |
| 3.1.1.20 | tannin acyl hydrolase | - |
Enterobacter sp. |
| 3.1.1.20 | tannin acyl hydrolase | - |
Serratia ficaria |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 30 | - |
- |
Rhodococcus sp. |
| 3.1.1.20 | 30 | 40 | - |
Lactiplantibacillus plantarum |
| 3.1.1.20 | 30 | 40 | - |
Selenomonas ruminantium |
| 3.1.1.20 | 35 | - |
- |
Escherichia coli |
| 3.1.1.20 | 35 | - |
- |
Serratia ficaria |
| 3.1.1.20 | 40 | - |
- |
Bacillus subtilis |
| 3.1.1.20 | 40 | - |
- |
Bacillus cereus |
| 3.1.1.20 | 40 | - |
- |
Klebsiella pneumoniae |
| 3.1.1.20 | 40 | - |
- |
Enterobacter sp. |
| 3.1.1.20 | 50 | - |
- |
Enterobacter cloacae |
| 3.1.1.20 | 50 | - |
- |
Lysinibacillus sphaericus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 25 | 45 | - |
Escherichia coli |
| 3.1.1.20 | 30 | 70 | - |
Bacillus subtilis |
| 3.1.1.20 | 30 | 80 | - |
Lysinibacillus sphaericus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 4.5 | - |
- |
Bacillus cereus |
| 3.1.1.20 | 5 | - |
- |
Bacillus subtilis |
| 3.1.1.20 | 5 | - |
- |
Lysinibacillus sphaericus |
| 3.1.1.20 | 5 | 8 | - |
Lactiplantibacillus plantarum |
| 3.1.1.20 | 5.5 | - |
- |
Enterobacter cloacae |
| 3.1.1.20 | 5.5 | - |
- |
Klebsiella pneumoniae |
| 3.1.1.20 | 5.5 | - |
- |
Enterobacter sp. |
| 3.1.1.20 | 6 | - |
- |
Rhodococcus sp. |
| 3.1.1.20 | 7 | - |
- |
Escherichia coli |
| 3.1.1.20 | 7 | - |
- |
Selenomonas ruminantium |
| 3.1.1.20 | 8.9 | - |
- |
Serratia ficaria |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 3 | 8 | - |
Bacillus subtilis |
| 3.1.1.20 | 3 | 8 | - |
Lysinibacillus sphaericus |
| 3.1.1.20 | 4.5 | 5 | - |
Bacillus cereus |
| 3.1.1.20 | 5.5 | 8 | - |
Escherichia coli |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.1.20 | Bacillus subtilis | isoelectric focusing | - |
4.4 |
| 3.1.1.20 | Selenomonas ruminantium | isoelectric focusing | - |
6.58 |
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