Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chin, Y.W.; Park, J.B.; Park, Y.C.; Kim, K.H.; Seo, J.H.
    Metabolic engineering of Corynebacterium glutamicum to produce GDP-L-fucose from glucose and mannose (2013), Bioprocess Biosyst. Eng., 36, 749-756.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.281 gene rmd, constitutive recombinant overexpression in Spodoptera frugiperda Sf9 cells or in Trichoplusia ni High Five cells under the transcriptional control of the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) immediate early 1 (ie1) promoter and via baculovirus transfection. Rmd to blocks the production of GDP-L-fucose and glycoprotein fucosylation because GDP-L-fucose is required as the donor substrate for this process Pseudomonas aeruginosa
4.2.1.47 expressed in Corynebacterium glutamicum strain ATCC 13032 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.281 additional information GDP-L-fucose biosynthesis in eukaryotic cells is reduced by expression of bacterial Rmd, which converts GDP-4-dehydro-6-deoxy-D-mannose to GDP-D-rhamnose, assessing a vector-based glycoengineering strategy for blocking glycoprotein fucosylation in insect cells, overview. N-glycan profiling by MALDI-TOF mass spectrometry Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.47 42000
-
 x * 42000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.281 GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H + H+ Pseudomonas aeruginosa
-
 GDP-D-rhamnose + NAD(P)+
-
 ?
4.2.1.47 GDP-alpha-D-mannose Escherichia coli
-
 GDP-4-dehydro-alpha-D-rhamnose + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.281 Pseudomonas aeruginosa Q9HTB6
-
-
4.2.1.47 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.281 GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H + H+
-
 Pseudomonas aeruginosa GDP-D-rhamnose + NAD(P)+
-
 ?
4.2.1.47 GDP-alpha-D-mannose
-
 Escherichia coli GDP-4-dehydro-alpha-D-rhamnose + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.47 ? x * 42000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.1.1.281 GDP-6-deoxy-D-mannose reductase
-
 Pseudomonas aeruginosa
1.1.1.281 RMD
-
 Pseudomonas aeruginosa
4.2.1.47 GDP-D-mannose-4,6-dehydratase
-
 Escherichia coli
4.2.1.47 GMD
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.281 NAD(P)H
-
 Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
1.1.1.281 metabolism the enzyme consumes the immediate precursor to GDP-L-fucose to produce GDP-D-rhamnose, which might be a dead-end product in recombinant insect cells Pseudomonas aeruginosa