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Literature summary extracted from

  • Shin, H.Y.; Lee, J.H.; Lee, J.Y.; Han, Y.O.; Han, M.J.; Kim, D.H.
    Purification and characterization of ginsenoside Ra-hydrolyzing beta-D-xylosidase from Bifidobacterium breve K-110, a human intestinal anaerobic bacterium (2003), Biol. Pharm. Bull., 26, 1170-1173.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 Cu2+ weak inhibition Bifidobacterium breve
3.2.1.37 Mn2+ weak inhibition Bifidobacterium breve
3.2.1.37 p-chloromercuryphenylsulfonic acid potent inhibitor Bifidobacterium breve

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 1.54
-
 4-nitrophenyl beta-D-xylopyranoside in 50 mM phosphate buffer (pH 7.0), at 37°C Bifidobacterium breve
3.2.1.37 4.76
-
 ginsenoside Ra2 in 50 mM phosphate buffer (pH 7.0), at 37°C Bifidobacterium breve
3.2.1.37 5.56
-
 ginsenoside Ra1 in 50 mM phosphate buffer (pH 7.0), at 37°C Bifidobacterium breve

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 49000
-
 x * 49000, SDS-PAGE Bifidobacterium breve

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Bifidobacterium breve B1P195
-
-
3.2.1.37 Bifidobacterium breve K-110 B1P195
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 ammonium sulfate precipitation, QAE cellulose column chromatography, butyl Toyopearl column chromatography, hydroxyapatite column chromatography, and Q Sepharose column chromatography Bifidobacterium breve

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 0.05
-
 crude extract, in 50 mM phosphate buffer (pH 7.0), at 37°C Bifidobacterium breve
3.2.1.37 51.82
-
 1036fold purified enzyme, in 50 mM phosphate buffer (pH 7.0), at 37°C Bifidobacterium breve

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O 100% activity Bifidobacterium breve 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O 100% activity Bifidobacterium breve K-110 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.37 ginsenoside Ra1 + H2O 5.2% activity compared to 4-nitrophenyl beta-D-xylopyranoside Bifidobacterium breve ginsenoside Rb2 + D-xylose
-
 ?
3.2.1.37 ginsenoside Ra1 + H2O 5.2% activity compared to 4-nitrophenyl beta-D-xylopyranoside Bifidobacterium breve K-110 ginsenoside Rb2 + D-xylose
-
 ?
3.2.1.37 ginsenoside Ra2 + H2O 4.2% activity compared to 4-nitrophenyl beta-D-xylopyranoside Bifidobacterium breve ginsenoside Rc + D-xylose
-
 ?
3.2.1.37 ginsenoside Ra2 + H2O 4.2% activity compared to 4-nitrophenyl beta-D-xylopyranoside Bifidobacterium breve K-110 ginsenoside Rc + D-xylose
-
 ?
3.2.1.37 additional information the enzyme does not act on 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-glucopyranoside, maltose, cellobiose, ginsenoside Rb2, and ginsenoside Rc Bifidobacterium breve ?
-
 ?
3.2.1.37 additional information the enzyme does not act on 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-glucopyranoside, maltose, cellobiose, ginsenoside Rb2, and ginsenoside Rc Bifidobacterium breve K-110 ?
-
 ?
3.2.1.37 xylan + H2O 0.4% activity compared to 4-nitrophenyl beta-D-xylopyranoside Bifidobacterium breve ?
-
 ?
3.2.1.37 xylan + H2O 0.4% activity compared to 4-nitrophenyl beta-D-xylopyranoside Bifidobacterium breve K-110 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 ? x * 49000, SDS-PAGE Bifidobacterium breve

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 5
-
 at 37°C Bifidobacterium breve

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.37 Bifidobacterium breve isoelectric focusing
-
 4.2