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Literature summary extracted from

  • Hopwood, E.M.; Ahmed, D.; Aitken, S.M.
    A role for glutamate-333 of Saccharomyces cerevisiae cystathionine gamma-lyase as a determinant of specificity (2014), Biochim. Biophys. Acta, 1844, 465-472.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.1 E333A site-directed mutagenesis of the active-site residue, pH optimum 7.8-8.6, the mutant shows increased KM for L-cystathionine up to 17fold, and 2.5fold for O-acetyl-L-serine Saccharomyces cerevisiae
4.4.1.1 E333D site-directed mutagenesis of the active-site residue, pH optimum 7.2-8.0, the mutant shows increased KM for L-cystathionine up to 17fold, and 2.5fold for O-acetyl-L-serine Saccharomyces cerevisiae
4.4.1.1 E333Q site-directed mutagenesis of the active-site residue, pH optimum 7.6-8.4, the mutant shows increased KM for L-cystathionine up to 17fold, and 2.5fold for O-acetyl-L-serine Saccharomyces cerevisiae
4.4.1.1 E48A site-directed mutagenesis of the active-site residue, pH optimum 7.6-8.4 Saccharomyces cerevisiae
4.4.1.1 E48A/E333A site-directed mutagenesis of the active-site residues, pH optimum 8.4-9.2 Saccharomyces cerevisiae
4.4.1.1 E48D site-directed mutagenesis of the active-site residue, pH optimum 7.07.8 Saccharomyces cerevisiae
4.4.1.1 E48D/E333D site-directed mutagenesis of the active-site residues, pH optimum 7.6-8.4 Saccharomyces cerevisiae
4.4.1.1 E48Q site-directed mutagenesis of the active-site residue, pH optimum 7.4-8.2 Saccharomyces cerevisiae
4.4.1.1 E48Q/E333Q site-directed mutagenesis of the active-site residues, pH optimum 7.9-8.7 Saccharomyces cerevisiae
4.4.1.1 additional information pH optima of the mutant enzymes vary from wild-type enzyme, overview Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.1 additional information
-
 additional information Michaelis-Menten kinetics Saccharomyces cerevisiae
4.4.1.1 0.09
-
 L-cysteine pH 7.2, 25°C, recombinant wild-type enzyme Saccharomyces cerevisiae
4.4.1.1 0.11
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48A Saccharomyces cerevisiae
4.4.1.1 0.18
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48Q Saccharomyces cerevisiae
4.4.1.1 0.4
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48D Saccharomyces cerevisiae
4.4.1.1 0.4
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48Q/E333Q Saccharomyces cerevisiae
4.4.1.1 0.5
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48A/E333A Saccharomyces cerevisiae
4.4.1.1 0.5
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48D/E333D Saccharomyces cerevisiae
4.4.1.1 0.7
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E333Q Saccharomyces cerevisiae
4.4.1.1 0.7
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48D/E333D Saccharomyces cerevisiae
4.4.1.1 0.71
-
 L-cystathionine pH 7.2, 25°C, recombinant wild-type enzyme Saccharomyces cerevisiae
4.4.1.1 0.76
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E48A Saccharomyces cerevisiae
4.4.1.1 0.87
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E48D Saccharomyces cerevisiae
4.4.1.1 0.9
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E333D Saccharomyces cerevisiae
4.4.1.1 0.9
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E48Q Saccharomyces cerevisiae
4.4.1.1 1.2
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E333A Saccharomyces cerevisiae
4.4.1.1 1.4
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48D Saccharomyces cerevisiae
4.4.1.1 1.5
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48A Saccharomyces cerevisiae
4.4.1.1 1.5
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48Q/E333Q Saccharomyces cerevisiae
4.4.1.1 1.8
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E333D Saccharomyces cerevisiae
4.4.1.1 2.2
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E333A Saccharomyces cerevisiae
4.4.1.1 2.5
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant wild-type enzyme Saccharomyces cerevisiae
4.4.1.1 2.9
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48A/E333A Saccharomyces cerevisiae
4.4.1.1 4.4
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48Q Saccharomyces cerevisiae
4.4.1.1 5.3
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E333Q Saccharomyces cerevisiae
4.4.1.1 12
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E333A Saccharomyces cerevisiae
4.4.1.1 12
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E333D Saccharomyces cerevisiae
4.4.1.1 13
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E333Q Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.1 L-cystathionine + H2O Saccharomyces cerevisiae
-
 L-cysteine + 2-oxobutanoate + NH3
-
 r
4.4.1.1 L-cysteine + H2O Saccharomyces cerevisiae
-
 H2S + pyruvate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.1 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.1 L-cystathionine + H2O
-
 Saccharomyces cerevisiae L-cysteine + 2-oxobutanoate + NH3
-
 r
4.4.1.1 L-cysteine + H2O
-
 Saccharomyces cerevisiae H2S + pyruvate + NH3
-
 ?
4.4.1.1 additional information Glu333 of cystathionine gamma-lyase acts as a determinant of specificity, it interacts with the distal amine moiety of L-cystathionine, which is not present in the alternative substrate O-acetyl-L-serine. Catalytic efficiency of the yeast enzyme for alpha,gamma-elimination of O-succinyl-L-homoserine, which possesses a distal carboxylate, but lacks an amino group, is 300fold lower than that of the physiological L-cystathionine substrate and 260fold higher than that of L-Hcys, which lacks both distal polar moieties. Proposed polar contacts of the yCGL active site that interact with or are influenced by E48 and E333 overview Saccharomyces cerevisiae ?
-
 ?
4.4.1.1 O-acetyl-L-serine + H2O
-
 Saccharomyces cerevisiae acetate + pyruvate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.1 cgl
-
 Saccharomyces cerevisiae
4.4.1.1 yCGL
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.1 25
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.1 0.0035
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48D/E333D Saccharomyces cerevisiae
4.4.1.1 0.0045
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E333D Saccharomyces cerevisiae
4.4.1.1 0.0047
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48A/E333A Saccharomyces cerevisiae
4.4.1.1 0.0053
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48D Saccharomyces cerevisiae
4.4.1.1 0.0058
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E333A Saccharomyces cerevisiae
4.4.1.1 0.0092
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48Q/E333Q Saccharomyces cerevisiae
4.4.1.1 0.0097
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant wild-type enzyme Saccharomyces cerevisiae
4.4.1.1 0.0102
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E333Q Saccharomyces cerevisiae
4.4.1.1 0.0124
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48Q Saccharomyces cerevisiae
4.4.1.1 0.0142
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48A Saccharomyces cerevisiae
4.4.1.1 0.029
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48D/E333D Saccharomyces cerevisiae
4.4.1.1 0.09
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48A Saccharomyces cerevisiae
4.4.1.1 0.11
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48A/E333A Saccharomyces cerevisiae
4.4.1.1 0.15
-
 L-cysteine pH 7.2, 25°C, recombinant wild-type enzyme Saccharomyces cerevisiae
4.4.1.1 0.16
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48Q/E333Q Saccharomyces cerevisiae
4.4.1.1 0.18
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48Q Saccharomyces cerevisiae
4.4.1.1 0.24
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E333D Saccharomyces cerevisiae
4.4.1.1 0.32
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48D Saccharomyces cerevisiae
4.4.1.1 0.48
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E333Q Saccharomyces cerevisiae
4.4.1.1 0.5
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E333Q Saccharomyces cerevisiae
4.4.1.1 0.54
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E48A Saccharomyces cerevisiae
4.4.1.1 0.78
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E333A Saccharomyces cerevisiae
4.4.1.1 0.98
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E333D Saccharomyces cerevisiae
4.4.1.1 1.29
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E48Q Saccharomyces cerevisiae
4.4.1.1 1.3
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E333A Saccharomyces cerevisiae
4.4.1.1 1.4
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E48D Saccharomyces cerevisiae
4.4.1.1 1.51
-
 L-cystathionine pH 7.2, 25°C, recombinant wild-type enzyme Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.1 7.2 7.8 wild-type enzyme Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.1 pyridoxal 5'-phosphate
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
4.4.1.1 metabolism cystathionine gamma-lyase catalyzes the hydrolysis of L-cystathionine in the second step of the reverse transsulfuration pathway, which converts L-homocysteine to L-Cys Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.4.1.1 0.0016
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48A/E333A Saccharomyces cerevisiae
4.4.1.1 0.0019
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E333Q Saccharomyces cerevisiae
4.4.1.1 0.0026
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E333A Saccharomyces cerevisiae
4.4.1.1 0.0028
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48Q Saccharomyces cerevisiae
4.4.1.1 0.0037
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E48A/E333A Saccharomyces cerevisiae
4.4.1.1 0.0038
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant wild-type enzyme and mutant E48D Saccharomyces cerevisiae
4.4.1.1 0.005
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E333D Saccharomyces cerevisiae
4.4.1.1 0.005
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48D/E333D Saccharomyces cerevisiae
4.4.1.1 0.006
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48Q/E333Q Saccharomyces cerevisiae
4.4.1.1 0.0088
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E48D/E333D Saccharomyces cerevisiae
4.4.1.1 0.01
-
 O-acetyl-L-serine pH 7.2, 25°C, recombinant mutant E48A Saccharomyces cerevisiae
4.4.1.1 0.0169
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E48Q/E333Q Saccharomyces cerevisiae
4.4.1.1 0.036
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E333Q Saccharomyces cerevisiae
4.4.1.1 0.06
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48D/E333D Saccharomyces cerevisiae
4.4.1.1 0.065
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E333A Saccharomyces cerevisiae
4.4.1.1 0.082
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E333D Saccharomyces cerevisiae
4.4.1.1 0.13
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E333D Saccharomyces cerevisiae
4.4.1.1 0.21
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48A/E333A Saccharomyces cerevisiae
4.4.1.1 0.31
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48Q/E333Q Saccharomyces cerevisiae
4.4.1.1 0.49
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E333Q Saccharomyces cerevisiae
4.4.1.1 0.71
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E48A Saccharomyces cerevisiae
4.4.1.1 0.72
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48D Saccharomyces cerevisiae
4.4.1.1 0.8
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48A Saccharomyces cerevisiae
4.4.1.1 0.81
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E333A Saccharomyces cerevisiae
4.4.1.1 1.02
-
 L-cysteine pH 7.2, 25°C, recombinant mutant E48Q Saccharomyces cerevisiae
4.4.1.1 1.4
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E48Q Saccharomyces cerevisiae
4.4.1.1 1.59
-
 L-cystathionine pH 7.2, 25°C, recombinant mutant E48D Saccharomyces cerevisiae
4.4.1.1 1.7
-
 L-cysteine pH 7.2, 25°C, recombinant wild-type enzyme Saccharomyces cerevisiae
4.4.1.1 2.1
-
 L-cystathionine pH 7.2, 25°C, recombinant wild-type enzyme Saccharomyces cerevisiae