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Literature summary extracted from

  • Hashimoto, W.; Maruyama, Y.; Nakamichi, Y.; Mikami, B.; Murata, K.
    Crystal structure of Pedobacter heparinus heparin lyase Hep III with the active site in a deep cleft (2014), Biochemistry, 53, 777-786.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.2.8 isoform Hep III, to 2.2 A resolution. The enzyme comprises an N-terminal alpha/alpha-barrel domain and a C-terminal antiparallel beta-sheet domain as its basic scaffold. Isoform Hep III exhibits an open form compared with the closed form of Hep II. An active site of Hep III is located in the deep cleft at the interface between its two domains Pedobacter heparinus

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.8 E237A 40.8% of wild-type activitiy Pedobacter heparinus
4.2.2.8 F423A 42.4% of wild-type activitiy Pedobacter heparinus
4.2.2.8 H241A 29.7% of wild-type activitiy Pedobacter heparinus
4.2.2.8 H424A 0.3% of wild-type activitiy Pedobacter heparinus
4.2.2.8 I29V/L657S 42.7% of wild-type activitiy Pedobacter heparinus
4.2.2.8 N240A 3.1% of wild-type activitiy Pedobacter heparinus
4.2.2.8 Q238A 88.5% of wild-type activitiy Pedobacter heparinus
4.2.2.8 W350A 27.7% of wild-type activitiy Pedobacter heparinus
4.2.2.8 Y294F 2.8% of wild-type activitiy Pedobacter heparinus
4.2.2.8 Y450F 5.6% of wild-type activitiy Pedobacter heparinus
4.2.2.8 Y590 7.7% of wild-type activitiy Pedobacter heparinus

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.8 Pedobacter heparinus Q59289
-
-
4.2.2.8 Pedobacter heparinus DSM 2366 Q59289
-
-

Synonyms

EC Number Synonyms Comment Organism
4.2.2.8 Hep III
-
 Pedobacter heparinus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.8 39.4
-
 melting temperature, presence of 1 mM heparin tetrasaccharide Pedobacter heparinus
4.2.2.8 40.5
-
 melting temperature, presence of 0.1 mM heparin tetrasaccharide Pedobacter heparinus
4.2.2.8 43.9
-
 melting temperature, presence of 0.1 mM heparin disaccharide Pedobacter heparinus
4.2.2.8 44.3
-
 melting temperature, presence of 0.1 mM N-acetyl glucosamine Pedobacter heparinus
4.2.2.8 45.2
-
 melting temperature, presence of 0.1 mM gellan tetrasaccharide Pedobacter heparinus
4.2.2.8 45.44
-
 melting temperature Pedobacter heparinus