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Literature summary extracted from

  • Bovell, A.M.; Warncke, K.
    The structural model of Salmonella typhimurium ethanolamine ammonia-lyase directs a rational approach to the assembly of the functional [(EutB-EutC)2]3 oligomer from isolated subunits (2013), Biochemistry, 52, 1419-1428.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.1.7 tris-(2-carboxyethyl) phosphine leads to 25fold increase in kcat Salmonella enterica subsp. enterica serovar Typhimurium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.7 expressed as a His-taggd fusion protein Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.7 0.022
-
ethanolamine reconstituted EAL (from EutB and EutC subunits), pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.7 0.0316
-
ethanolamine purified EAL, pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.7 30000
-
SDS-PAGE, EutC monomer Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.7 48000
-
SDS-PAGE, EutB monomer Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.7 494000
-
native PAGE, EAL oligomer Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.7 Salmonella enterica subsp. enterica serovar Typhimurium P19264 and P19265 heavy chain: P19264, light chain: P19265
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.7 ethanolamine
-
Salmonella enterica subsp. enterica serovar Typhimurium acetaldehyde + NH3
-
?

Subunits

EC Number Subunits Comment Organism
4.3.1.7 oligomer EutB and EutC monomers form the EutB-EutC heterodimer. Two heterodimers form a (EutB-EutC)2 homodimer, and three (EutB-EutC)2 homodimers form the biologically active oligomer. The model predicts that (EutB2)3 oligomer assembly occurs from isolated EutB, and that this hexameric structure templates the formation of the complete, native [(EutB-EutC)2]3 oligomer Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
4.3.1.7 EAL
-
Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.7 ethanolamine ammonia-lyase
-
Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.7 25
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.1.7 16.7
-
ethanolamine reconstituted EAL (from EutB and EutC subunits), pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.7 22.7
-
ethanolamine purified EAL, pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.7 7.5
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.1.7 720
-
ethanolamine purified EAL, pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.7 730
-
ethanolamine reconstituted EAL (from EutB and EutC subunits), pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium