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Literature summary extracted from

  • McIntyre, N.R.; Franco, R.; Shelnutt, J.A.; Ferreira, G.C.
    Nickel(II) chelatase variants directly evolved from murine ferrochelatase: porphyrin distortion and kinetic mechanism (2011), Biochemistry, 50, 1535-1544.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
4.98.1.1 F323L the mutant exhibits an enhanced Ni2+-chelatase activity over that of the parent wild type ferrochelatase Rattus norvegicus
4.98.1.1 S143T the mutant exhibits an enhanced Ni2+-chelatase activity over that of the parent wild type ferrochelatase Rattus norvegicus
4.98.1.1 S143T/F323L the mutant exhibits an enhanced Ni2+-chelatase activity over that of the parent wild type ferrochelatase Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.98.1.1 additional information no Ni2+ substrate inhibition of ferrochelatase is observed Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.98.1.1 0.00026
-
 protoporphyrin IX wild type enzyme, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.00029
-
 protoporphyrin IX mutant enzyme S143T, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.00123
-
 protoporphyrin IX mutant enzyme F323L, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.00172
-
 protoporphyrin IX wild type enzyme, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.00226
-
 Fe2+ wild type enzyme, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.00243
-
 protoporphyrin IX mutant enzyme S143T/F323L, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.00291
-
 Fe2+ mutant enzyme S143T, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.00547
-
 protoporphyrin IX mutant enzyme F323L, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.00558
-
 protoporphyrin IX mutant enzyme S143T/F323L, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.00728
-
 Fe2+ mutant enzyme S143T/F323L, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.00831
-
 protoporphyrin IX mutant enzyme S143T, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.00895
-
 Fe2+ mutant enzyme F323L, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.02206
-
 Ni2+ wild type enzyme, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.03218
-
 Ni2+ mutant enzyme F323L, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.06521
-
 Ni2+ mutant enzyme S143T, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.1037
-
 Ni2+ mutant enzyme S143T/F323L, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.98.1.1 protoporphyrin IX + Fe2+ Rattus norvegicus
-
 protoheme IX + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.98.1.1 Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.98.1.1 protoporphyrin IX + Fe2+
-
 Rattus norvegicus protoheme IX + 2 H+
-
 ?
4.98.1.1 protoporphyrin IX + Ni2+
-
 Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.98.1.1 protoheme lyase
-
 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.98.1.1 0.0125
-
 protoporphyrin IX wild type enzyme, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.142
-
 protoporphyrin IX wild type enzyme, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.212
-
 protoporphyrin IX mutant enzyme F323L, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.44
-
 protoporphyrin IX mutant enzyme S143T/F323L, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 0.59
-
 protoporphyrin IX mutant enzyme S143T, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 1.21
-
 protoporphyrin IX mutant enzyme S143T, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 1.6
-
 protoporphyrin IX mutant enzyme F323L, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 1.9
-
 protoporphyrin IX mutant enzyme S143T/F323L, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.98.1.1 0.56
-
 Ni2+ wild type enzyme, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 1.605
-
 Ni2+ mutant enzyme F323L, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 4.23
-
 Ni2+ mutant enzyme S143T/F323L, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 9.05
-
 Ni2+ mutant enzyme S143T, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 47.3
-
 protoporphyrin IX wild type enzyme, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 62.5
-
 Fe2+ wild type enzyme, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 82.3
-
 protoporphyrin IX wild type enzyme, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 145.7
-
 protoporphyrin IX mutant enzyme S143T, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 173.3
-
 protoporphyrin IX mutant enzyme F323L, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 179
-
 Fe2+ mutant enzyme F323L, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 180
-
 protoporphyrin IX mutant enzyme S143T/F323L, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 261.3
-
 Fe2+ mutant enzyme S143T/F323L, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 292.8
-
 protoporphyrin IX mutant enzyme F323L, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 340.5
-
 protoporphyrin IX mutant enzyme S143T/F323L, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 416.8
-
 Fe2+ mutant enzyme S143T, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus
4.98.1.1 2015
-
 protoporphyrin IX mutant enzyme S143T, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C Rattus norvegicus