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Literature summary extracted from

  • Mahlstedt, S.; Fielding, E.N.; Moore, B.S.; Walsh, C.T.
    Prephenate decarboxylases: a new prephenate-utilizing enzyme family that performs nonaromatizing decarboxylation en route to diverse secondary metabolites (2010), Biochemistry, 49, 9021-9023.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.100 expression in Escherichia coli with an N-terminal His6 tag Planktothrix agardhii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.100 0.17
-
 prephenate pH and temperature not specified in the publication Planktothrix agardhii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.100 prephenate Planktothrix agardhii the enzyme is involved in the biosynthesis of the nonribosomal glycopeptides aeruginoside 126A and B. These natural products contain the unusual amino acid residue 2-carboxy-6-hydroxy-octahydroindole 3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate + CO2 the product is non-enzymatically isomerized to the thermodynamically favored conjugated exocyclic diene 3-((1R,4R)-4-hydroxycyclohexa-2-enylidene)-2-oxopropanoate ?
4.1.1.100 prephenate Salinispora tropica the enzyme is involved in the biosynthetic pathway to the proteasome inhibitor salinosporamide A. SalX is relevant to the production of the nonproteinogenic amino acid building block cyclohexenylalanine 3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.100 Planktothrix agardhii
-
-
-
4.1.1.100 Salinispora tropica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.100
-
 Planktothrix agardhii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.100 prephenate
-
 Salinispora tropica 3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate + CO2
-
 ?
4.1.1.100 prephenate
-
 Planktothrix agardhii 3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate + CO2
-
 ?
4.1.1.100 prephenate the enzyme is involved in the biosynthesis of the nonribosomal glycopeptides aeruginoside 126A and B. These natural products contain the unusual amino acid residue 2-carboxy-6-hydroxy-octahydroindole Planktothrix agardhii 3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate + CO2 the product is non-enzymatically isomerized to the thermodynamically favored conjugated exocyclic diene 3-((1R,4R)-4-hydroxycyclohexa-2-enylidene)-2-oxopropanoate ?
4.1.1.100 prephenate the enzyme is involved in the biosynthetic pathway to the proteasome inhibitor salinosporamide A. SalX is relevant to the production of the nonproteinogenic amino acid building block cyclohexenylalanine Salinispora tropica 3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.100 aerD
-
 Planktothrix agardhii
4.1.1.100 SalX
-
 Salinispora tropica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.100 4.1
-
 prephenate pH and temperature not specified in the publication Planktothrix agardhii

General Information

EC Number General Information Comment Organism
4.1.1.100 physiological function the enzyme is involved in the biosynthesis of the nonribosomal glycopeptides aeruginoside 126A and B. These natural products contain the unusual amino acid residue 2-carboxy-6-hydroxy-octahydroindole Planktothrix agardhii
4.1.1.100 physiological function the enzyme is involved in the biosynthetic pathway to the proteasome inhibitor salinosporamide A. SalX is relevant to the production of the nonproteinogenic amino acid building block cyclohexenylalanine Salinispora tropica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.100 12.2
-
 prephenate pH and temperature not specified in the publication Salinispora tropica
4.1.1.100 24
-
 prephenate pH and temperature not specified in the publication Planktothrix agardhii