EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.99.B5 | D229N | mutant enzyme without acarviosyl transferase activity and 4-alpha-glucanotransferase activity | Actinoplanes sp. |
2.4.99.B5 | D328N | mutant enzyme without acarviosyl transferase activity and 4-alpha-glucanotransferase activity | Actinoplanes sp. |
2.4.99.B5 | E257Q | mutant enzyme without acarviosyl transferase activity and 4-alpha-glucanotransferase activity | Actinoplanes sp. |
2.4.99.B5 | G140H | mutation changes acarviosyl transferase into an enzyme with 4-alpha-glucanotransferase activity. This mutation introduces cyclodextrin-forming activity into acarviosyl transferase, converting 2% of starch into cyclodextrins | Actinoplanes sp. |
2.4.99.B5 | G140H | mutation decreases acarviosyl transferase activity, 4-alpha-glucanotransferase activity is strongly enhanced | Actinoplanes sp. |
2.4.99.B5 | G140H/Q327H | mutation changes acarviosyl transferase into an enzyme with 4-alpha-glucanotransferase activity. This mutation introduces cyclodextrin-forming activity into acarviosyl transferase, converting 1.2% of starch into cyclodextrins | Actinoplanes sp. |
2.4.99.B5 | G140H/Q327H/T329M | mutation changes acarviosyl transferase into an enzyme with 4-alpha-glucanotransferase activity. This mutation introduces cyclodextrin-forming activity into acarviosyl transferase, converting 7.4% of starch into cyclodextrins | Actinoplanes sp. |
2.4.99.B5 | Q327H | mutation decreases acarviosyl transferase activity, 4-alpha-glucanotransferase activity is reduced | Actinoplanes sp. |
2.4.99.B5 | T329M | mutation decreases acarviosyl transferase activity, 4-alpha-glucanotransferase activity is strongly enhanced | Actinoplanes sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.99.B5 | Actinoplanes sp. | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.99.B5 | 2 | - |
pH 6.5, 30°C | Actinoplanes sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.99.B5 | acarbose + maltotriose | - |
Actinoplanes sp. | acarviosyl-alpha-(1->4)-maltotriose + maltose | - |
? | |
2.4.99.B5 | additional information | the enzyme is inactive on starch | Actinoplanes sp. | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.99.B5 | ATASE | - |
Actinoplanes sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.99.B5 | 30 | - |
assay at | Actinoplanes sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.99.B5 | 6.5 | - |
assay at | Actinoplanes sp. |