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Literature summary extracted from

  • Shen, B.; Hutchinson, C.R.
    Tetracenomycin F2 cyclase: intramolecular aldol condensation in the biosynthesis of tetracenomycin C in Streptomyces glaucescens (1993), Biochemistry, 32, 11149-11154.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.154 0.121
-
 tetracenomycin F2 at pH 8.0 and 30°C Streptomyces glaucescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.154 12500
-
 3 * 12500, SDS-PAGE Streptomyces glaucescens
4.2.1.154 12728
-
 3 * 12728, calculated from amino acid sequence Streptomyces glaucescens
4.2.1.154 37500
-
 gel filtration Streptomyces glaucescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.154 tetracenomycin F2 Streptomyces glaucescens at pH 8.0 or higher, the enzyme catalyzes the cyclization of tetracenomycin F2 to tetracenomycin F1, while below pH 6.5, the enzyme catalyzes the cyclization of tetracenomycin F2 to 9-decarboxy-tetracenomycin F1 tetracenomycin F1 + H2O
-
 ?
4.2.1.154 tetracenomycin F2 Streptomyces glaucescens WMH1068 at pH 8.0 or higher, the enzyme catalyzes the cyclization of tetracenomycin F2 to tetracenomycin F1, while below pH 6.5, the enzyme catalyzes the cyclization of tetracenomycin F2 to 9-decarboxy-tetracenomycin F1 tetracenomycin F1 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.154 Streptomyces glaucescens
-
-
-
4.2.1.154 Streptomyces glaucescens WMH1068
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.154 ammonium sulfate precipitation, Mono Q column chromatography, phenyl-Superose gel filtration, Superose 6 gel filtration, and Sephacryl S-200 gel filtration Streptomyces glaucescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.154 0.000571
-
 cell-free extract, at pH 8.0 and 30°C Streptomyces glaucescens
4.2.1.154 0.309
-
 after 541fold purification, at pH 8.0 and 30°C Streptomyces glaucescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.154 tetracenomycin F2 at pH 8.0 or higher, the enzyme catalyzes the cyclization of tetracenomycin F2 to tetracenomycin F1, while below pH 6.5, the enzyme catalyzes the cyclization of tetracenomycin F2 to 9-decarboxy-tetracenomycin F1 Streptomyces glaucescens tetracenomycin F1 + H2O
-
 ?
4.2.1.154 tetracenomycin F2 at pH 8.0 or higher, the enzyme catalyzes the cyclization of tetracenomycin F2 to tetracenomycin F1, while below pH 6.5, the enzyme catalyzes the cyclization of tetracenomycin F2 to 9-decarboxy-tetracenomycin F1 Streptomyces glaucescens WMH1068 tetracenomycin F1 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.154 homotrimer 3 * 12500, SDS-PAGE Streptomyces glaucescens
4.2.1.154 homotrimer 3 * 12728, calculated from amino acid sequence Streptomyces glaucescens

Synonyms

EC Number Synonyms Comment Organism
4.2.1.154 Tcm F2 cyclase
-
 Streptomyces glaucescens
4.2.1.154 tcmI
-
 Streptomyces glaucescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.154 5 8.5
-
 Streptomyces glaucescens