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Literature summary extracted from

  • Miyanaga, A.; Fujisawa, S.; Furukawa, N.; Arai, K.; Nakajima, M.; Taguchi, H.
    The crystal structure of D-mandelate dehydrogenase reveals its distinct substrate and coenzyme recognition mechanisms from those of 2-ketopantoate reductase (2013), Biochem. Biophys. Res. Commun., 439, 109-114.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.345 expression in Escherichia coli Enterococcus faecium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.345 crystals are grown from a 1:1 mixture of a protein solution (10 mg/ml in 10 mM Tris–HCl (pH 7.5)) and a reservoir solution (0.085 M HEPES-Na (pH 7.5), 0.17 M ammonium acetate and 22.5% PEG8000) using the hanging-drop vapor diffusion method at 25°C. The overall structure shows that the enzyme has a similar fold to 2-ketopantoate reductase, which catalyzes the conversion of 2-ketopantoate to D-pantoate using NADP+ as a coenzyme. They share conserved catalytic residues, indicating that D-mandelate dehydrogenase ManDH2 has the same reaction mechanism as 2-ketopantoate reductase. However, D-mandelate dehydrogenase ManDH2 exhibits significant structural variations in the coenzyme and substrate binding sites compared to 2-ketopantoate reductase. These structural observations can explain their different coenzyme and substrate specificities Enterococcus faecium

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.345 K187A mutant has completely lost its activity Enterococcus faecium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.169 (R)-4-dehydropantoate + NADPH + H+ Enterococcus faecalis
-
 (R)-pantoate + NADP+
-
 ?
1.1.1.169 (R)-4-dehydropantoate + NADPH + H+ Enterococcus faecalis IAM10071
-
 (R)-pantoate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.169 Enterococcus faecalis E3USM3
-
-
1.1.1.169 Enterococcus faecalis IAM10071 E3USM3
-
-
1.1.1.345 Enterococcus faecium E3USM3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.345
-
 Enterococcus faecium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.169 (R)-4-dehydropantoate + NADPH + H+
-
 Enterococcus faecalis (R)-pantoate + NADP+
-
 ?
1.1.1.169 (R)-4-dehydropantoate + NADPH + H+
-
 Enterococcus faecalis IAM10071 (R)-pantoate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.169 2-ketopantoate reductase
-
 Enterococcus faecalis
1.1.1.169 KPR
-
 Enterococcus faecalis
1.1.1.345 D-mandelate dehydrogenase misleading Enterococcus faecium
1.1.1.345 ManDH2
-
 Enterococcus faecium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.169 NADPH
-
 Enterococcus faecalis