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Literature summary extracted from

  • Rashamuse, K.; Ronneburg, T.; Sanyika, W.; Mathiba, K.; Mmutlane, E.; Brady, D.
    Metagenomic mining of feruloyl esterases from termite enteric flora (2014), Appl. Microbiol. Biotechnol., 98, 727-737.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 gene fae1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, heterologous expression in Escherichia coli strain BL21(DE3) uncultured bacterium
3.1.1.73 gene fae2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, heterologous expression in Escherichia coli strain BL21(DE3) uncultured bacterium
3.1.1.73 gene fae3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, heterologous expression in Escherichia coli strain BL21(DE3) uncultured bacterium
3.1.1.73 gene fae4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, heterologous expression in Escherichia coli strain BL21(DE3) uncultured bacterium
3.1.1.73 gene fae5, DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, heterologous expression in Escherichia coli strain BL21(DE3) uncultured bacterium
3.1.1.73 gene fae6, DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, heterologous expression in Escherichia coli strain BL21(DE3) uncultured bacterium
3.1.1.73 gene fae7, DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, heterologous expression in Escherichia coli strain BL21(DE3) uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 uncultured bacterium
-
 isolated from midgut of Trinervitermes trinervoides, collected from Komatipoort, Mpumalanga, South Africa, gene fae5
-
3.1.1.73 uncultured bacterium M9VR53 FAE4; isolated from midgut of Trinervitermes trinervoides, collected from Komatipoort, Mpumalanga, South Africa, gene fae4
-
3.1.1.73 uncultured bacterium M9VTY8 FAE3; isolated from midgut of Trinervitermes trinervoides, collected from Komatipoort, Mpumalanga, South Africa, gene fae3
-
3.1.1.73 uncultured bacterium M9VUD6 FAE2; isolated from midgut of Trinervitermes trinervoides, collected from Komatipoort, Mpumalanga, South Africa, gene fae2
-
3.1.1.73 uncultured bacterium M9VUE0 FAE7; isolated from midgut of Trinervitermes trinervoides, collected from Komatipoort, Mpumalanga, South Africa, gene fae7
-
3.1.1.73 uncultured bacterium M9VY17 FAE1; isolated from midgut of Trinervitermes trinervoides, collected from Komatipoort, Mpumalanga, South Africa, gene fae1
-
3.1.1.73 uncultured bacterium M9VY20 FAE6; isolated from midgut of Trinervitermes trinervoides, collected from Komatipoort, Mpumalanga, South Africa, gene fae6
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 ethyl ferulate + H2O
-
 uncultured bacterium ferulate + ethanol
-
 ?
3.1.1.73 methyl 4-coumarate + H2O low activity uncultured bacterium 4-coumarate + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O low activity uncultured bacterium caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 uncultured bacterium ferulate + methanol
-
 ?
3.1.1.73 methyl sinapinate + H2O preferred substrate uncultured bacterium sinapate + methanol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAE1
-
 uncultured bacterium
3.1.1.73 FAE2
-
 uncultured bacterium
3.1.1.73 FAE3
-
 uncultured bacterium
3.1.1.73 FAE4
-
 uncultured bacterium
3.1.1.73 FAE5
-
 uncultured bacterium
3.1.1.73 FAE6
-
 uncultured bacterium
3.1.1.73 FAE7
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 40
-
-
 uncultured bacterium
3.1.1.73 50
-
-
 uncultured bacterium
3.1.1.73 60
-
-
 uncultured bacterium
3.1.1.73 70
-
-
 uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 20 50 activity range uncultured bacterium
3.1.1.73 30 60 activity range uncultured bacterium
3.1.1.73 40 60 activity range uncultured bacterium
3.1.1.73 50 90 activity range uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 30
-
 recombinant enzyme, half-life is over 8 h uncultured bacterium
3.1.1.73 30 50 recombinant enzyme, half-life is over 8 h uncultured bacterium
3.1.1.73 30 70 recombinant enzyme, half-life is over 8 h uncultured bacterium
3.1.1.73 30 40 recombinant enzyme, half-life is over 8 h uncultured bacterium
3.1.1.73 30 60 recombinant enzyme, half-life is over 8 h uncultured bacterium
3.1.1.73 40
-
 recombinant enzyme, half-life is 7 h uncultured bacterium
3.1.1.73 50
-
 recombinant enzyme, half-life is 1.5 h uncultured bacterium
3.1.1.73 50
-
 recombinant enzyme, half-life is below 1 h uncultured bacterium
3.1.1.73 60
-
 recombinant enzyme, half-life is 6 h uncultured bacterium
3.1.1.73 60
-
 recombinant enzyme, inactivation uncultured bacterium
3.1.1.73 70
-
 recombinant enzyme, half-life is below 1 h uncultured bacterium
3.1.1.73 70
-
 recombinant enzyme, inactivation uncultured bacterium
3.1.1.73 80
-
 recombinant enzyme, half-life is 2.5 h uncultured bacterium
3.1.1.73 80
-
 recombinant enzyme, inactivation uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 7
-
-
 uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 6 8 over 60% of maximal activity within this range uncultured bacterium
3.1.1.73 6 7 over 60% of maximal activity within this range uncultured bacterium
3.1.1.73 7 8 over 60% of maximal activity within this range uncultured bacterium

General Information

EC Number General Information Comment Organism
3.1.1.73 evolution the termite-derived isozymes form a distinct subclass of feroyl esterases uncultured bacterium
3.1.1.73 additional information the catalytic triad is formed by Ser121, Asp215 and His244 uncultured bacterium
3.1.1.73 additional information the catalytic triad is formed by Ser123, Asp212 and His241 uncultured bacterium
3.1.1.73 additional information the catalytic triad is formed by Ser124, Asp215 and His244 uncultured bacterium
3.1.1.73 additional information the catalytic triad is formed by Ser125, Asp216 and His245 uncultured bacterium
3.1.1.73 additional information the catalytic triad is formed by Ser126, Asp215 and His244 uncultured bacterium
3.1.1.73 additional information the catalytic triad is formed by Ser128, Asp215 and His244 uncultured bacterium
3.1.1.73 additional information the catalytic triad is formed by Ser131, Asp222 and His251 uncultured bacterium