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Literature summary extracted from

  • Jimenez, N.; Barcenilla, J.M.; de Felipe, F.L.; de Las Rivas, B.; Munoz, R.
    Characterization of a bacterial tannase from Streptococcus gallolyticus UCN34 suitable for tannin biodegradation (2014), Appl. Microbiol. Biotechnol., 98, 6329-6337.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.20 DMSO 144% activity at 1 mM Streptococcus gallolyticus
3.1.1.20 Tween 80 119% activity at 1 mM Streptococcus gallolyticus

Application

EC Number Application Comment Organism
3.1.1.20 degradation TanSg1 is a tannase with potential industrial interest regarding the biodegradation of tannin waste or its bioconversion into biologically active products Streptococcus gallolyticus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.20 expressed in Escherichia coli BL21(DE3) cells Streptococcus gallolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 2-mercaptoethanol 16% residual activity at 1 mM Streptococcus gallolyticus
3.1.1.20 EDTA 80% residual activity at 1 mM Streptococcus gallolyticus
3.1.1.20 Hg2+ 6% residual activity at 1 mM Streptococcus gallolyticus
3.1.1.20 K+ 69% residual activity at 1 mM Streptococcus gallolyticus
3.1.1.20 Mg2+ 65% residual activity at 1 mM Streptococcus gallolyticus
3.1.1.20 Triton X-100 78% residual activity at 1 mM Streptococcus gallolyticus
3.1.1.20 Urea 35% residual activity at 1 mM Streptococcus gallolyticus
3.1.1.20 Zn2+ 10% residual activity at 1 mM Streptococcus gallolyticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.20 Ca2+ 120% activity at 1 mM Streptococcus gallolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.20 52980
-
 x * 52980, calculated from amino acid sequence Streptococcus gallolyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 gallotannin + H2O Streptococcus gallolyticus
-
 gallate + D-glucose + ?
-
 ?
3.1.1.20 tannic acid + H2O Streptococcus gallolyticus
-
 gallate + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Streptococcus gallolyticus
-
-
-
3.1.1.20 Streptococcus gallolyticus UCN34
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 Talon Superflow resin column chromatography Streptococcus gallolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 butyl 4-hydroxybenzoate + H2O
-
 Streptococcus gallolyticus butanol + 4-hydroxybenzoate
-
 ?
3.1.1.20 epigallocatechin gallate + H2O
-
 Streptococcus gallolyticus epigallocatechin + gallate
-
 ?
3.1.1.20 ethyl 3,4-dihydroxybenzoate + H2O
-
 Streptococcus gallolyticus ethanol + 3,4-dihydroxybenzoate
-
 ?
3.1.1.20 ethyl 3,4-dihydroxybenzoate + H2O
-
 Streptococcus gallolyticus UCN34 ethanol + 3,4-dihydroxybenzoate
-
 ?
3.1.1.20 ethyl 3,5-dihydroxybenzoate + H2O
-
 Streptococcus gallolyticus ethanol + 3,5-dihydroxybenzoate
-
 ?
3.1.1.20 ethyl 4-hydroxybenzoate + H2O
-
 Streptococcus gallolyticus ethanol + 4-hydroxybenzoate
-
 ?
3.1.1.20 ethyl 4-hydroxybenzoate + H2O
-
 Streptococcus gallolyticus UCN34 ethanol + 4-hydroxybenzoate
-
 ?
3.1.1.20 ethyl benzoate + H2O
-
 Streptococcus gallolyticus ethanol + benzoate
-
 ?
3.1.1.20 ethyl benzoate + H2O
-
 Streptococcus gallolyticus UCN34 ethanol + benzoate
-
 ?
3.1.1.20 ethyl gallate + H2O
-
 Streptococcus gallolyticus gallate + ethanol
-
 ?
3.1.1.20 ferulic ethyl ester + H2O
-
 Streptococcus gallolyticus ethanol + ferulate
-
 ?
3.1.1.20 ferulic methyl ester + H2O
-
 Streptococcus gallolyticus methanol + ferulate
-
 ?
3.1.1.20 gallotannin + H2O
-
 Streptococcus gallolyticus gallate + D-glucose + ?
-
 ?
3.1.1.20 lauryl gallate + H2O
-
 Streptococcus gallolyticus gallate + lauric acid
-
 ?
3.1.1.20 methyl 2,4-dihydroxybenzoate + H2O
-
 Streptococcus gallolyticus methanol + 2,4-dihydroxybenzoate
-
 ?
3.1.1.20 methyl 4-hydroxybenzoate + H2O
-
 Streptococcus gallolyticus methanol + 4-hydroxybenzoate
-
 ?
3.1.1.20 methyl benzoate + H2O
-
 Streptococcus gallolyticus methanol + benzoate
-
 ?
3.1.1.20 methyl benzoate + H2O
-
 Streptococcus gallolyticus UCN34 methanol + benzoate
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Streptococcus gallolyticus gallate + methanol
-
 ?
3.1.1.20 methyl gentisate + H2O
-
 Streptococcus gallolyticus methanol + gentisate
-
 ?
3.1.1.20 methyl salicylate + H2O
-
 Streptococcus gallolyticus methanol + salicylate
-
 ?
3.1.1.20 methyl vanillate + H2O
-
 Streptococcus gallolyticus methanol + vanillate
-
 ?
3.1.1.20 methyl vanillate + H2O
-
 Streptococcus gallolyticus UCN34 methanol + vanillate
-
 ?
3.1.1.20 propyl 4-hydroxybenzoate + H2O
-
 Streptococcus gallolyticus propanol + 4-hydroxybenzoate
-
 ?
3.1.1.20 propyl gallate + H2O
-
 Streptococcus gallolyticus gallate + propanol
-
 ?
3.1.1.20 protocatechuic acid ethyl ester + H2O
-
 Streptococcus gallolyticus ethanol + protocatechuate
-
 ?
3.1.1.20 tannic acid + H2O
-
 Streptococcus gallolyticus gallate + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 ? x * 52980, calculated from amino acid sequence Streptococcus gallolyticus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 GALLO_1609 locus name Streptococcus gallolyticus
3.1.1.20 tannin acyl hydrolase
-
 Streptococcus gallolyticus
3.1.1.20 TanSg1
-
 Streptococcus gallolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 45
-
-
 Streptococcus gallolyticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.20 4 65 active between 4 and 65°C Streptococcus gallolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.20 45 65 the protein dramatically loses its activity after 30 min at 65°C. However, the enzyme keeps more than 70% activity after 30 min of incubation at 55°C. The enzyme shows about 50 and 25% of the maximal activity after 6 and 18 h of incubation at 45°C, respectively Streptococcus gallolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 7
-
-
 Streptococcus gallolyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.20 6 8 highly active at pH 6.0-8.0. At pH 5.0, the enzyme shows less than 20% activity. At pH 3.0 and 10.0, the enzyme completely loses its activity Streptococcus gallolyticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.20 Streptococcus gallolyticus calculated from amino acid sequence
-
 5.1