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Literature summary extracted from

  • Uzura, A.; Nomoto, F.; Sakoda, A.; Nishimoto, Y.; Kataoka, M.; Shimizu, S.
    Stereoselective synthesis of (R)-3-quinuclidinol through asymmetric reduction of 3-quinuclidinone with 3-quinuclidinone reductase of Rhodotorula rubra (2009), Appl. Microbiol. Biotechnol., 83, 617-626.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.B51 pharmacology stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals Rhodotorula mucilaginosa
1.1.1.B51 synthesis stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals Rhodotorula mucilaginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B51 an expression vector, pWKLQ, which contains the full length 3-quinuclidinone reductase gene is constructed. Using Escherichia coli cells coexpressing the 3-quinuclidinone reductase and glucose dehydrogenase (cofactor regeneration enzyme) genes, 618 mM 3-quinuclidinone is almost stiochiometrically converted to (R)-3-quinuclidinol with an >99.9% enantiomeric excess within 21 h of reaction Rhodotorula mucilaginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.B51 4-mercuribenzoate 1 mM, complete inhibition Rhodotorula mucilaginosa
1.1.1.B51 5,5'-dithiobis(2-nitrobenzoate) 1 mM, 15% inhibition Rhodotorula mucilaginosa
1.1.1.B51 Hg2+ 1 mM, complete inhibition Rhodotorula mucilaginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.B51 0.19
-
 NADPH pH 7.0, temperature not specified in the publication Rhodotorula mucilaginosa
1.1.1.B51 145
-
 3-quinuclidinone pH 7.0, temperature not specified in the publication Rhodotorula mucilaginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.B51 30000
-
 3 or 4 * 3 * 30000, SDS-PAGE Rhodotorula mucilaginosa
1.1.1.B51 93000
-
 gel filtration Rhodotorula mucilaginosa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B51 Rhodotorula mucilaginosa B9ZZZ6
-
-
1.1.1.B51 Rhodotorula mucilaginosa JCM 3782 B9ZZZ6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.B51
-
 Rhodotorula mucilaginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.B51 341
-
 pH 7.0, temperature not specified in the publication Rhodotorula mucilaginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B51 3-quinuclidinone + NADPH + H+
-
 Rhodotorula mucilaginosa (R)-3-quinuclidinol + NADP+
-
 ?
1.1.1.B51 3-quinuclidinone + NADPH + H+
-
 Rhodotorula mucilaginosa JCM 3782 (R)-3-quinuclidinol + NADP+
-
 ?
1.1.1.B51 ethyl 4-chloro-3-oxobutanoate + NADPH + H+
-
 Rhodotorula mucilaginosa ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
 ?
1.1.1.B51 ethyl 4-chloro-3-oxobutanoate + NADPH + H+
-
 Rhodotorula mucilaginosa JCM 3782 ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
 ?
1.1.1.B51 ketopantoyl lactone + NADPH + H+ the optical purity of pantoyl lactone is only 17.2% ee for L-isomer Rhodotorula mucilaginosa pantoyl lactone + NADP+
-
 ?
1.1.1.B51 ketopantoyl lactone + NADPH + H+ the optical purity of pantoyl lactone is only 17.2% ee for L-isomer Rhodotorula mucilaginosa JCM 3782 pantoyl lactone + NADP+
-
 ?
1.1.1.B51 additional information no activity with: acetaldehyde, propionaldehyde, heptanal, glyoxal, benzaldehyde, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, 2-chlorobenzaldehyde, 3-chlorobenzaldehyde, pyridine-3-aldehyde, 2,4-pentanedione, 3-methylcyclohexanone, 4-methylcyclohexanone, 4-ethylcyclohexanone, isatin, p-toluquinone, alpha-naphthoquinone, menadione, ketoisophorone, levodione, 4-piperidone hydrochloride monohydrate, 1-methyl-4-piperidone, and tropinone Rhodotorula mucilaginosa ?
-
 ?
1.1.1.B51 additional information no activity with: acetaldehyde, propionaldehyde, heptanal, glyoxal, benzaldehyde, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, 2-chlorobenzaldehyde, 3-chlorobenzaldehyde, pyridine-3-aldehyde, 2,4-pentanedione, 3-methylcyclohexanone, 4-methylcyclohexanone, 4-ethylcyclohexanone, isatin, p-toluquinone, alpha-naphthoquinone, menadione, ketoisophorone, levodione, 4-piperidone hydrochloride monohydrate, 1-methyl-4-piperidone, and tropinone Rhodotorula mucilaginosa JCM 3782 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.B51 ? 3 or 4 * 3 * 30000, SDS-PAGE Rhodotorula mucilaginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B51 40
-
-
 Rhodotorula mucilaginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.B51 6.5 7.5
-
 Rhodotorula mucilaginosa
1.1.1.B51 7
-
 assay at Rhodotorula mucilaginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B51 NADPH highly specific for NADPH as a coenzyme Rhodotorula mucilaginosa