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Literature summary extracted from

  • Lu, J.; Takahashi, A.; Ueda, S.
    3-Hydroxybutyrate oligomer hydrolase and 3-hydroxybutyrate dehydrogenase participate in intracellular polyhydroxybutyrate and polyhydroxyvalerate degradation in Paracoccus denitrificans (2014), Appl. Environ. Microbiol., 80, 986-993.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.30 expressed in Escherichia coli BL21(DE3) cells Paracoccus denitrificans
3.1.1.22 expressed in Escherichia coli JM109 cells Paracoccus denitrificans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.22 diisopropylfluorophosphate completely inhibited by 1 mM diisopropylfluorophosphate Paracoccus denitrificans
3.1.1.22 iodoacetamide 74.8% residual activity at 1 mM Paracoccus denitrificans
3.1.1.22 N-ethylmaleimide 74.8% residual activity at 1 mM Paracoccus denitrificans
3.1.1.22 phenylmethylsulfonyl fluoride 27.4% residual activity at 1 mM Paracoccus denitrificans
3.1.1.22 SDS 61.1% residual activity at 0.05% (w/v) Paracoccus denitrificans
3.1.1.22 Triton X-100 67.3% residual activity at 0.05% (v/v) Paracoccus denitrificans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.30 27200
-
-
 Paracoccus denitrificans
1.1.1.30 29000
-
 4 * 29000, SDS-PAGE Paracoccus denitrificans
1.1.1.30 120000
-
 gel filtration Paracoccus denitrificans
3.1.1.22 31000
-
 4 * 31000, SDS-PAGE Paracoccus denitrificans
3.1.1.22 120000
-
 gel filtration Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+ Paracoccus denitrificans
-
 acetoacetate + NADH + H+
-
 ?
1.1.1.30 (R)-3-hydroxybutanoate + NAD+ Paracoccus denitrificans ATCC 17741
-
 acetoacetate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.30 Paracoccus denitrificans
-
-
-
1.1.1.30 Paracoccus denitrificans ATCC 17741
-
-
-
3.1.1.22 Paracoccus denitrificans V5YT97
-
-
3.1.1.22 Paracoccus denitrificans PD01 V5YT97
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.30 Ni-NTA column chromatography, and TSK gel G3000SW gel filtration Paracoccus denitrificans
3.1.1.22 ammonium sulfate precipitation, Ni-NTA column chromatography, and TSK gel G3000SW gel filtration Paracoccus denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.22 0.48
-
 with 3-(3-hydroxypentanoyloxy)pentanoate as substrate, at pH 8.0 and 30°C Paracoccus denitrificans
3.1.1.22 0.67
-
 with 3-(3-hydroxybutanoyloxy)butanoate as substrate, at pH 8.0 and 30°C Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Paracoccus denitrificans acetoacetate + NADH + H+
-
 ?
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Paracoccus denitrificans ATCC 17741 acetoacetate + NADH + H+
-
 ?
1.1.1.30 3-hydroxyvalerate + NAD+
-
 Paracoccus denitrificans 3-oxovalerate + NADH + H+
-
 ?
1.1.1.30 3-hydroxyvalerate + NAD+
-
 Paracoccus denitrificans ATCC 17741 3-oxovalerate + NADH + H+
-
 ?
3.1.1.22 3-(3-hydroxybutanoyloxy)butanoate + H2O
-
 Paracoccus denitrificans 3-hydroxybutanoate
-
 ?
3.1.1.22 3-(3-hydroxybutanoyloxy)butanoate + H2O
-
 Paracoccus denitrificans PD01 3-hydroxybutanoate
-
 ?
3.1.1.22 3-(3-hydroxypentanoyloxy)pentanoate + H2O
-
 Paracoccus denitrificans 3-hydroxypentanoate
-
 ?
3.1.1.22 3-(3-hydroxypentanoyloxy)pentanoate + H2O
-
 Paracoccus denitrificans PD01 3-hydroxypentanoate
-
 ?
3.1.1.22 4-nitrophenyl butyrate + H2O
-
 Paracoccus denitrificans 4-nitrophenol + butanoate
-
 ?
3.1.1.22 4-nitrophenyl butyrate + H2O
-
 Paracoccus denitrificans PD01 4-nitrophenol + butanoate
-
 ?
3.1.1.22 4-nitrophenyl pentanoate + H2O
-
 Paracoccus denitrificans 4-nitrophenol + pentanoate
-
 ?
3.1.1.22 4-nitrophenyl pentanoate + H2O
-
 Paracoccus denitrificans PD01 4-nitrophenol + pentanoate
-
 ?
3.1.1.22 additional information pHB and PHV granules are not depolymerized by the enzyme Paracoccus denitrificans ?
-
 ?
3.1.1.22 additional information pHB and PHV granules are not depolymerized by the enzyme Paracoccus denitrificans PD01 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.30 homotetramer 4 * 29000, SDS-PAGE Paracoccus denitrificans
1.1.1.30 homotetramer 4 * 27200, calculated from amino acid sequence Paracoccus denitrificans
3.1.1.22 homotetramer 4 * 31000, SDS-PAGE Paracoccus denitrificans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.30 HBD
-
 Paracoccus denitrificans
3.1.1.22 3-hydroxybutyrate oligomer hydrolase
-
 Paracoccus denitrificans
3.1.1.22 PhaZc
-
 Paracoccus denitrificans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.22 50
-
-
 Paracoccus denitrificans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.22 20 60 more than 50% activity between 20-60°C Paracoccus denitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.22 8
-
-
 Paracoccus denitrificans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.22 5 9 more than 50% activity between pH 5.0 and 9.0 Paracoccus denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.30 NAD+
-
 Paracoccus denitrificans