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Literature summary extracted from

  • Martinez, V.; de la Pena, F.; Garcia-Hidalgo, J.; de la Mata, I.; Garcia, J.L.; Prieto, M.A.
    Identification and biochemical evidence of a medium-chain-length polyhydroxyalkanoate depolymerase in the Bdellovibrio bacteriovorus predatory hydrolytic arsenal (2012), Appl. Environ. Microbiol., 78, 6017-6026.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.76 EDTA 10% activation at 10 mM Bdellovibrio bacteriovorus
3.1.1.76 sodium cholate 30% activation at 0.02 mM, 18% at 0.2 mM Bdellovibrio bacteriovorus

Application

EC Number Application Comment Organism
3.1.1.76 synthesis the enzyme migt be used as biocatalyst for the production of enantiopure hydroxyalkanoic acids and oligomers as building blocks for the synthesis of biobased polymers Bdellovibrio bacteriovorus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.76 orf Bd3709, recombinant extracellular expression in different hypersecretor Tol-pal mutant strains of Escherichia coli and Pseudomonas putida, e.g. in Escherichia coli strain DH10B(pIZBd1) periplasm or Pseudomonas putida strain KT2442, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)(pETBd1) Bdellovibrio bacteriovorus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.76 cetyltrimethylammonium bromide 88% inhibition at 0.05 vol% Bdellovibrio bacteriovorus
3.1.1.76 deoxycholate 84% inhibition at 0.05 vol% Bdellovibrio bacteriovorus
3.1.1.76 dithiothreitol 45% inhibition at 10 mM DTT Bdellovibrio bacteriovorus
3.1.1.76 additional information not affected by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide treatment, and by 1 mM cyclodextrin Bdellovibrio bacteriovorus
3.1.1.76 phenylmethylsulfonyl fluoride 10% inhibition at 1 mM, 90% inhibition at 10 mM Bdellovibrio bacteriovorus
3.1.1.76 SDS 47% inhibition at 0.05 vol% Bdellovibrio bacteriovorus
3.1.1.76 sodium cholate 57% inhibition at 2 mM Bdellovibrio bacteriovorus
3.1.1.76 Triton X-100 90% inhibition at 0.01 vol% Bdellovibrio bacteriovorus
3.1.1.76 Tween 80 92% inhibition at 0.05 vol%, 79% inhibition at 0.005 vol% Bdellovibrio bacteriovorus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.76 extracellular
-
 Bdellovibrio bacteriovorus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.76 additional information no effect by 1 mM MgCl2 or CaCl2 Bdellovibrio bacteriovorus
3.1.1.76 NaCl a high ionic strength of 0.5 M NaCl in the reaction mixture is required for optimal activity Bdellovibrio bacteriovorus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.76 additional information Bdellovibrio bacteriovorus the enzyme is an endoexohydrolase that cleaves both large and small polyhydroxyalkanoate molecules, producing mainly dimers but also monomers and trimers. The enzyme specifically degrades medium-chain-length polyhydroxyalkanoates and is inactive toward short-chain-length polyhydroxyalkanoates like polyhydroxybutyrate ?
-
 ?
3.1.1.76 additional information Bdellovibrio bacteriovorus HD100 the enzyme is an endoexohydrolase that cleaves both large and small polyhydroxyalkanoate molecules, producing mainly dimers but also monomers and trimers. The enzyme specifically degrades medium-chain-length polyhydroxyalkanoates and is inactive toward short-chain-length polyhydroxyalkanoates like polyhydroxybutyrate ?
-
 ?
3.1.1.76 polyhydroxyalkanoate latex + H2O Bdellovibrio bacteriovorus
-
 ?
-
 ?
3.1.1.76 polyhydroxyalkanoate latex + H2O Bdellovibrio bacteriovorus HD100
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.76 Bdellovibrio bacteriovorus
-
 orf Bd3709
-
3.1.1.76 Bdellovibrio bacteriovorus HD100
-
 orf Bd3709
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.76 recombinant extracellular enzyme by hydrophobic interaction chromatography, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)(pETBd1) by nickel affinity chromatography Bdellovibrio bacteriovorus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.76 55 560 purified recombinant His-tagged enzme, pH 8.0, 37°C, different assay methods Bdellovibrio bacteriovorus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.76 additional information the enzyme is an endoexohydrolase that cleaves both large and small polyhydroxyalkanoate molecules, producing mainly dimers but also monomers and trimers. The enzyme specifically degrades medium-chain-length polyhydroxyalkanoates and is inactive toward short-chain-length polyhydroxyalkanoates like polyhydroxybutyrate Bdellovibrio bacteriovorus ?
-
 ?
3.1.1.76 additional information the enzyme is an endoexohydrolase that cleaves both large and small polyhydroxyalkanoate molecules, producing mainly dimers but also monomers and trimers. The enzyme specifically degrades medium-chain-length polyhydroxyalkanoates and is inactive toward short-chain-length polyhydroxyalkanoates like polyhydroxybutyrate Bdellovibrio bacteriovorus HD100 ?
-
 ?
3.1.1.76 polyhydroxyalkanoate latex + H2O
-
 Bdellovibrio bacteriovorus ?
-
 ?
3.1.1.76 polyhydroxyalkanoate latex + H2O
-
 Bdellovibrio bacteriovorus HD100 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.76 extracellular MCL-PHA depolymerase
-
 Bdellovibrio bacteriovorus
3.1.1.76 MCL-PHA depolymerase
-
 Bdellovibrio bacteriovorus
3.1.1.76 medium-chain-length polyhydroxyalkanoate depolymerase
-
 Bdellovibrio bacteriovorus
3.1.1.76 PhaZBd
-
 Bdellovibrio bacteriovorus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.76 37
-
-
 Bdellovibrio bacteriovorus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.76 4 50 activity range, highly active at 4-45°C, 29% of maximal activityy at 50°C Bdellovibrio bacteriovorus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.76 10
-
-
 Bdellovibrio bacteriovorus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.76 5 11 activity range, almost inactive at pH 12.0 Bdellovibrio bacteriovorus

General Information

EC Number General Information Comment Organism
3.1.1.76 evolution the enzyme belongs to the alpha/beta-hydrolase fold family Bdellovibrio bacteriovorus
3.1.1.76 additional information the enzyme has a typical serine hydrolase catalytic triad, serine-histidine-aspartic acid Bdellovibrio bacteriovorus