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Literature summary extracted from

  • Kaya, H.B.; Oezcan, B.; Sisecioglu, M.; Ozdemir, H.
    Purification of acetylcholinesterase by 9-amino-1,2,3,4-tetrahydroacridine from human erythrocytes (2013), Appl. Biochem. Biotechnol., 170, 198-209.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 6-methoxytacrine mixed competitive-uncompetitive inhibition Homo sapiens
3.1.1.7 tacrine i.e. 9-amino-1,2,3,4-tetrahydroacridine, mixed competitive-uncompetitive inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.7 0.025
-
 acetylthiocholine at pH 7.4 and 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.7 membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.7 70000
-
 x * 70000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.7 acetylcholine + H2O Homo sapiens
-
 choline + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.7 Sepharose 4B-L-tyrosine-tacrine affinity column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.7 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.7 0.0146
-
 hemolysate, at pH 7.4 and 37°C Homo sapiens
3.1.1.7 9.22
-
 after 658fold purification, at pH 7.4 and 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.7 acetylcholine + H2O
-
 Homo sapiens choline + acetate
-
 ?
3.1.1.7 acetylthiocholine + H2O
-
 Homo sapiens thiocholine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.7 ? x * 70000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.7 AChE
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.7 37
-
 optimum temperature at optimum pH Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.7 7.4
-
-
 Homo sapiens