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Literature summary extracted from

  • Zheng, Z.; Zhu, M.; He, Y.; Li, N.; Guo, T.; Chen, Y.; Wu, J.; Ying, H.; Xie, J.
    Gene cloning, expression, and characterization of a cyclic nucleotide phosphodiesterase from Arthrobacter sp. CGMCC 3584 (2013), Appl. Biochem. Biotechnol., 169, 2442-2456.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.17
-
 Arthrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.17 8-hydroxyquinoline 2 mM, 28% residual activity Arthrobacter sp.
3.1.4.17 ADP 1 mM, 56% residual activity Arthrobacter sp.
3.1.4.17 AMP 1 mM, 15% residual activity Arthrobacter sp.
3.1.4.17 ATP 1 mM, 46% residual activity Arthrobacter sp.
3.1.4.17 Ca2+ 2 mM, 69% residual activity Arthrobacter sp.
3.1.4.17 Cu2+ 2 mM, 27% residual activity Arthrobacter sp.
3.1.4.17 dipyridamole 1 mM, 71% residual activity Arthrobacter sp.
3.1.4.17 EDTA 2 mM, 44% residual activity Arthrobacter sp.
3.1.4.17 GDP 1 mM, 41% residual activity Arthrobacter sp.
3.1.4.17 GMP 1 mM, 31% residual activity Arthrobacter sp.
3.1.4.17 GTP 1 mM, 44% residual activity Arthrobacter sp.
3.1.4.17 NADH 1 mM, 76% residual activity Arthrobacter sp.
3.1.4.17 NADPH 1 mM, 25% residual activity Arthrobacter sp.
3.1.4.17 NaF 1 mM, 79% residual activity Arthrobacter sp.
3.1.4.17 theophylline 1 mM, 70% residual activity Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.17 6.82
-
 3',5'-cAMP pH 7.5, 30°C Arthrobacter sp.
3.1.4.17 12.82
-
 3',5'-cGMP pH 7.5, 30°C Arthrobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.17 Co2+ 2 mM, 212% of initial activity Arthrobacter sp.
3.1.4.17 Fe3+ 2 mM, 121% of initial activity Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.17 33600
-
 1 * 33600, calculated, 1 * 38000, SDS-PAGE of recombinant protein including His-tag Arthrobacter sp.
3.1.4.17 37800
-
 gel filtration Arthrobacter sp.
3.1.4.17 38000
-
 1 * 33600, calculated, 1 * 38000, SDS-PAGE of recombinant protein including His-tag Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.17 Arthrobacter sp. M9W6G9
-
-
3.1.4.17 Arthrobacter sp. CGMCC 3584 M9W6G9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.17 2',3'-cAMP + H2O
-
 Arthrobacter sp. 5'-AMP
-
 ?
3.1.4.17 2',3'-cAMP + H2O
-
 Arthrobacter sp. CGMCC 3584 5'-AMP
-
 ?
3.1.4.17 3',5'-cAMP + H2O
-
 Arthrobacter sp. 5'-AMP
-
 ?
3.1.4.17 3',5'-cAMP + H2O
-
 Arthrobacter sp. CGMCC 3584 5'-AMP
-
 ?
3.1.4.17 3',5'-cGMP + H2O
-
 Arthrobacter sp. 5'-GMP
-
 ?
3.1.4.17 3',5'-cGMP + H2O
-
 Arthrobacter sp. CGMCC 3584 5'-GMP
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.17 monomer 1 * 33600, calculated, 1 * 38000, SDS-PAGE of recombinant protein including His-tag Arthrobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.4.17 CpdA
-
 Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.17 45
-
-
 Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.17 7.5
-
-
 Arthrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.17 7 8 more than 80% of maximum activiy Arthrobacter sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.4.17 Arthrobacter sp. calculated
-
 5.5