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Literature summary extracted from

  • Lamppa, J.W.; Griswold, K.E.
    Alginate lyase exhibits catalysis-independent biofilm dispersion and antibiotic synergy (2013), Antimicrob. Agents Chemother., 57, 137-145.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.2.2.3 medicine alginate lyases are promising therapeutic candidates for treating mucoid Pseudomonas aeruginosa infections. In particular, the enzymes' ability to degrade alginate, a key component of mucoid biofilm matrix, has been the presumed mechanism by which they disrupt biofilms and enhance antibiotic efficacy, but invitro modeling shows that alginate lyase dispersion of Pseudomonas aeruginosa biofilms and enzyme synergy with tobramycin are completely decoupled from catalytic activity Pseudomonas aeruginosa
4.2.2.3 medicine alginate lyases are promising therapeutic candidates for treating mucoid Pseudomonas aeruginosa infections. In particular, the enzymes' ability to degrade alginate, a key component of mucoid biofilm matrix, has been the presumed mechanism by which they disrupt biofilms and enhance antibiotic efficacy, but invitro modeling shows that alginate lyase dispersion of Pseudomonas aeruginosa biofilms and enzyme synergy with tobramycin are completely decoupled from catalytic activity Sphingomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 expression of C-terminally His6-tagged A1-III Sphingomonas sp.
4.2.2.3 expression of C-terminally His6-tagged AlgL Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.3 H188A site-directed mutagenesis, the mutation switches the histidine 188 GTG codon to a CGC codon Sphingomonas sp.
4.2.2.3 Y242F site-directed mutagenesis, the mutation replaces the tyrosine 242 GTA codon with a GAA codon Sphingomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.3 additional information
-
 additional information pseudo Michaelis-Menten kinetics of native and recombinant His-tagged enzymes, overview Pseudomonas aeruginosa
4.2.2.3 additional information
-
 additional information pseudo Michaelis-Menten kinetics of native and recombinant His-tagged enzymes, overview Sphingomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Pseudomonas aeruginosa Q06749 gene algL
-
4.2.2.3 Sphingomonas sp. Q75WP3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 recombinant C-terminally His6-tagged A1-III by nickel affinity chromatography and dialysis, native enzyme by hydroxyapatite chromatography, ultrafiltration, and gel filtration Sphingomonas sp.
4.2.2.3 recombinant C-terminally His6-tagged AlgL by nickel affinity chromatography and dialysis, native enzyme by hydroxyapatite chromatography, ultrafiltration, and gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 additional information substrates are medium-viscosity brown seaweed alginate (BSWA) and bacterial (FRD1) alginate substrates from mucoid FRD1 biofilms Pseudomonas aeruginosa ?
-
 ?
4.2.2.3 additional information substrates are medium-viscosity brown seaweed alginate (BSWA) and bacterial (FRD1) alginate substrates from mucoid FRD1 biofilms Sphingomonas sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 A1-III
-
 Sphingomonas sp.
4.2.2.3 alginate lyase
-
 Pseudomonas aeruginosa
4.2.2.3 alginate lyase
-
 Sphingomonas sp.
4.2.2.3 AlgL
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 37
-
 assay at Pseudomonas aeruginosa
4.2.2.3 37
-
 assay at Sphingomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7
-
 assay at Pseudomonas aeruginosa
4.2.2.3 7
-
 assay at Sphingomonas sp.

General Information

EC Number General Information Comment Organism
4.2.2.3 metabolism alginate lyase dispersion of Pseudomonas aeruginosa biofilms and enzyme synergy with tobramycin are completely decoupled from catalytic activity, in vitro modeling, overview Pseudomonas aeruginosa
4.2.2.3 metabolism alginate lyase dispersion of Pseudomonas aeruginosa biofilms and enzyme synergy with tobramycin are completely decoupled from catalytic activity, in vitro modeling, overview Sphingomonas sp.