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Literature summary extracted from

  • Saa, L.; Mato, J.M.; Pavlov, V.
    Assays for methionine beta-lyase and S-adenosyl-L-homocysteine hydrolase based on enzymatic formation of CdS quantum dots in situ (2012), Anal. Chem., 84, 8961-8965.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.4.1.11 analysis the enzyme can be used for detection of S-adenosyl-L-homocysteine hydrolase activity and inhibition in a simple and robust fluorogenic enzymatic assay, method development, overview Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.11 43000
-
 4 * 43000, SDS-PAGE Pseudomonas putida
4.4.1.11 172000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.11 L-homocysteine Pseudomonas putida
-
 2-oxobutanoate + NH3 + hydrogen sulfide
-
 ?
4.4.1.11 L-homocysteine Pseudomonas putida ICR 3460
-
 2-oxobutanoate + NH3 + hydrogen sulfide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.11 Pseudomonas putida
-
-
-
4.4.1.11 Pseudomonas putida ICR 3460
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.11 native enzyme by anion exchange chromatography and gel filtration Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.11 commercial preparation
-
 Pseudomonas putida
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.11 L-homocysteine
-
 Pseudomonas putida 2-oxobutanoate + NH3 + hydrogen sulfide
-
 ?
4.4.1.11 L-homocysteine
-
 Pseudomonas putida 2-oxobutanoate + NH3 + hydrogen sulfide methionine gamma-lyase catalyzes the decomposition of homocysteine to hydrogen sulfide which forms fluorescent CdS nanoparticles in the presence of Cd(NO3)2. Hydrogen sulfide dissociates to yield S2? anions which readily interact with Cd2+ forming fluorescent CdS nanocrystals, assay method development and evaluation, overview ?
4.4.1.11 L-homocysteine
-
 Pseudomonas putida ICR 3460 2-oxobutanoate + NH3 + hydrogen sulfide
-
 ?
4.4.1.11 L-homocysteine
-
 Pseudomonas putida ICR 3460 2-oxobutanoate + NH3 + hydrogen sulfide methionine gamma-lyase catalyzes the decomposition of homocysteine to hydrogen sulfide which forms fluorescent CdS nanoparticles in the presence of Cd(NO3)2. Hydrogen sulfide dissociates to yield S2? anions which readily interact with Cd2+ forming fluorescent CdS nanocrystals, assay method development and evaluation, overview ?

Subunits

EC Number Subunits Comment Organism
4.4.1.11 tetramer 4 * 43000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
4.4.1.11 MGL
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.11 37
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.11 6.4
-
 assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.11 pyridoxal 5'-phosphate 4 mol of pyridoxal 5'-phosphate per mole of enzyme Pseudomonas putida

General Information

EC Number General Information Comment Organism
4.4.1.11 metabolism the enzyme is responsible for the alpha,beta,gamma-elimination and gamma,beta-replacement in analogues of L-methionione such as L-homocysteine, through degradation to keto acids, ammonia, and H2S in anaerobic bacteria and parasitic protozoa Pseudomonas putida