EC Number | Application | Comment | Organism |
---|---|---|---|
4.2.3.1 | analysis | continuous, coupled spectrophotometric threonine synthase assay. The sequential actions of threonine deaminase and hydroxyisocaproate dehydrogenase convert the L-Thr product of TS to alpha-ketobutyrate and then to 2-hydroxybutyrate, respectively, and are monitored as the decrease in absorbance at 340 nm resulting from the concomitant oxidation of beta-nicotinamide adenine dinucleotide to NAD+ by hydroxyisocaproate dehydrogenase | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.1 | 0.34 | - |
O-phospho-L-homoserine | pH 8.0, 25°C | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.1 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.1 | O-phospho-L-homoserine + H2O | - |
Escherichia coli | L-threonine + phosphate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.1 | 4 | - |
O-phospho-L-homoserine | pH 8.0, 25°C | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.3.1 | 8.7 | - |
- |
Escherichia coli |