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Literature summary extracted from

  • Morozova, E.A.; Kulikova, V.V.; Yashin, D.V.; Anufrieva, N.V.; Anisimova, N.Y.; Revtovich, S.V.; Kotlov, M.I.; Belyi, Y.F.; Pokrovsky, V.S.; Demidkina, T.V.
    Kinetic parameters and cytotoxic activity of recombinant methionine gamma-lyase from Clostridium tetani, Clostridium sporogenes, Porphyromonas gingivalis and Citrobacter freundii (2013), Acta Naturae, 5, 92-98.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.4.1.11 medicine the enzyme is of interest as an anticancer agent, since the growth of malignant cells of various origins (unlike the growth of normal cells) is accompanied by obligatory methionine utilization, and the enzyme is absent in mammalian cells. IC50 of MGL for several tumor cell cultures, overview Citrobacter freundii
4.4.1.11 medicine the enzyme is of interest as an anticancer agent, since the growth of malignant cells of various origins (unlike the growth of normal cells) is accompanied by obligatory methionine utilization, and the enzyme is absent in mammalian cells. IC50 of MGL for several tumor cell cultures, overview Clostridium sporogenes
4.4.1.11 medicine the enzyme is of interest as an anticancer agent, since the growth of malignant cells of various origins (unlike the growth of normal cells) is accompanied by obligatory methionine utilization, and the enzyme is absent in mammalian cells. IC50 of MGL for several tumor cell cultures, overview Clostridium tetani

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.11 expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Clostridium sporogenes
4.4.1.11 expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Clostridium tetani
4.4.1.11 expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Porphyromonas gingivalis
4.4.1.11 recombinant enzyme expression in Escherichia coli strain BL21 Citrobacter freundii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.11 additional information
-
 additional information steady-state kinetics for beta- and gamma-elimination reactions, overview Citrobacter freundii
4.4.1.11 additional information
-
 additional information steady-state kinetics for beta- and gamma-elimination reactions, overview Clostridium sporogenes
4.4.1.11 additional information
-
 additional information steady-state kinetics for beta- and gamma-elimination reactions, overview Clostridium tetani
4.4.1.11 additional information
-
 additional information steady-state kinetics for beta- and gamma-elimination reactions, overview Porphyromonas gingivalis
4.4.1.11 0.278
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
4.4.1.11 0.348
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
4.4.1.11 0.358
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
4.4.1.11 0.432
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
4.4.1.11 0.545
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
4.4.1.11 0.72
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
4.4.1.11 0.766
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
4.4.1.11 0.93
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
4.4.1.11 0.947
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
4.4.1.11 1.47
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
4.4.1.11 1.77
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
4.4.1.11 2.17
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
4.4.1.11 7.07
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Clostridium tetani
4.4.1.11 12.22
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
4.4.1.11 33.51
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.11 44040
-
 4 * 44040, His-tagged enzyme, sequence calcualtion Clostridium tetani
4.4.1.11 44080
-
 4 * 44080, His-tagged enzyme, sequence calcualtion Porphyromonas gingivalis
4.4.1.11 44360
-
 4 * 44360, His-tagged enzyme, sequence calcualtion Clostridium sporogenes
4.4.1.11 170000
-
 about, recombinant detagged enzyme, gel filtration Clostridium sporogenes
4.4.1.11 170000
-
 about, recombinant detagged enzyme, gel filtration Clostridium tetani
4.4.1.11 170000
-
 about, recombinant detagged enzyme, gel filtration Porphyromonas gingivalis
4.4.1.11 258000
-
 native enzyme, gel filtration Clostridium tetani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.11 L-methionine + H2O Citrobacter freundii gamma-elimination reaction methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-methionine + H2O Clostridium sporogenes gamma-elimination reaction methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-methionine + H2O Clostridium tetani gamma-elimination reaction methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-methionine + H2O Porphyromonas gingivalis gamma-elimination reaction methanethiol + NH3 + 2-oxobutanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.11 Citrobacter freundii
-
-
-
4.4.1.11 Clostridium sporogenes
-
-
-
4.4.1.11 Clostridium tetani
-
-
-
4.4.1.11 Porphyromonas gingivalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.11 recombinant enzyme from Escherichia coli strain BL21 Citrobacter freundii
4.4.1.11 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by thrombin and elimination by gel filtration Clostridium sporogenes
4.4.1.11 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by thrombin and elimination by gel filtration Clostridium tetani
4.4.1.11 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by thrombin and elimination by gel filtration Porphyromonas gingivalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.11 5
-
 purified His-tagged recombinant enzyme, pH 8.0, 30°C, substrate L-methionine Porphyromonas gingivalis
4.4.1.11 10.2
-
 purified recombinant enzyme, pH 8.0, 30°C, substrate L-methionine Citrobacter freundii
4.4.1.11 12.8
-
 purified His-tagged recombinant enzyme, pH 8.0, 30°C, substrate L-methionine Clostridium sporogenes
4.4.1.11 16.6
-
 purified His-tagged recombinant enzyme, pH 8.0, 30°C, substrate L-methionine Clostridium tetani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.11 L-methionine + H2O gamma-elimination reaction Citrobacter freundii methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-methionine + H2O gamma-elimination reaction Clostridium sporogenes methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-methionine + H2O gamma-elimination reaction Clostridium tetani methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-methionine + H2O gamma-elimination reaction Porphyromonas gingivalis methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-methionine sulfoxide + H2O gamma-elimination reaction Citrobacter freundii ?
-
 ?
4.4.1.11 L-methionine sulfoxide + H2O gamma-elimination reaction Clostridium sporogenes ?
-
 ?
4.4.1.11 L-methionine sulfoxide + H2O gamma-elimination reaction Clostridium tetani ?
-
 ?
4.4.1.11 L-methionine sulfoxide + H2O gamma-elimination reaction Porphyromonas gingivalis ?
-
 ?
4.4.1.11 additional information substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia Citrobacter freundii ?
-
 ?
4.4.1.11 additional information substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia Clostridium sporogenes ?
-
 ?
4.4.1.11 additional information substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia Clostridium tetani ?
-
 ?
4.4.1.11 additional information substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia Porphyromonas gingivalis ?
-
 ?
4.4.1.11 S-benzyl-L-cysteine + H2O beta-elimination reaction Citrobacter freundii ?
-
 ?
4.4.1.11 S-benzyl-L-cysteine + H2O beta-elimination reaction Clostridium sporogenes ?
-
 ?
4.4.1.11 S-benzyl-L-cysteine + H2O beta-elimination reaction Clostridium tetani ?
-
 ?
4.4.1.11 S-benzyl-L-cysteine + H2O beta-elimination reaction Porphyromonas gingivalis ?
-
 ?
4.4.1.11 S-ethyl-L-cysteine + H2O beta-elimination reaction Citrobacter freundii ?
-
 ?
4.4.1.11 S-ethyl-L-cysteine + H2O beta-elimination reaction Clostridium sporogenes ?
-
 ?
4.4.1.11 S-ethyl-L-cysteine + H2O beta-elimination reaction Clostridium tetani ?
-
 ?
4.4.1.11 S-ethyl-L-cysteine + H2O beta-elimination reaction Porphyromonas gingivalis ?
-
 ?
4.4.1.11 S-ethyl-L-homocysteine + H2O gamma-elimination reaction Citrobacter freundii ?
-
 ?
4.4.1.11 S-ethyl-L-homocysteine + H2O gamma-elimination reaction Clostridium sporogenes ?
-
 ?
4.4.1.11 S-ethyl-L-homocysteine + H2O gamma-elimination reaction Clostridium tetani ?
-
 ?
4.4.1.11 S-ethyl-L-homocysteine + H2O gamma-elimination reaction Porphyromonas gingivalis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.11 tetramer 4 * 44040, His-tagged enzyme, sequence calcualtion Clostridium tetani
4.4.1.11 tetramer 4 * 44080, His-tagged enzyme, sequence calcualtion Porphyromonas gingivalis
4.4.1.11 tetramer 4 * 44360, His-tagged enzyme, sequence calcualtion Clostridium sporogenes
4.4.1.11 tetramer dimer of dimers, 2 active sites, structure model Citrobacter freundii

Synonyms

EC Number Synonyms Comment Organism
4.4.1.11 MGL
-
 Citrobacter freundii
4.4.1.11 MGL
-
 Clostridium sporogenes
4.4.1.11 MGL
-
 Clostridium tetani
4.4.1.11 MGL
-
 Porphyromonas gingivalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.11 30
-
 assay at Citrobacter freundii
4.4.1.11 30
-
 assay at Clostridium sporogenes
4.4.1.11 30
-
 assay at Clostridium tetani
4.4.1.11 30
-
 assay at Porphyromonas gingivalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.11 2.7
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Clostridium tetani
4.4.1.11 3.84
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
4.4.1.11 3.9
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
4.4.1.11 5.05
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
4.4.1.11 5.8
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
4.4.1.11 5.89
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
4.4.1.11 6.3
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
4.4.1.11 6.7
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
4.4.1.11 7.05
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
4.4.1.11 7.08
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
4.4.1.11 8.05
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
4.4.1.11 8.5
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
4.4.1.11 9.86
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
4.4.1.11 10
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
4.4.1.11 12
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Clostridium tetani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.11 8
-
 assay at Citrobacter freundii
4.4.1.11 8
-
 assay at Clostridium sporogenes
4.4.1.11 8
-
 assay at Clostridium tetani
4.4.1.11 8
-
 assay at Porphyromonas gingivalis

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.11 pyridoxal 5'-phosphate
-
 Citrobacter freundii
4.4.1.11 pyridoxal 5'-phosphate
-
 Clostridium sporogenes
4.4.1.11 pyridoxal 5'-phosphate
-
 Clostridium tetani
4.4.1.11 pyridoxal 5'-phosphate
-
 Porphyromonas gingivalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.4.1.11 0.2
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
4.4.1.11 0.382
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Clostridium tetani
4.4.1.11 0.413
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
4.4.1.11 2.2
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
4.4.1.11 3.71
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
4.4.1.11 3.94
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
4.4.1.11 4.13
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
4.4.1.11 9.83
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
4.4.1.11 10.8
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
4.4.1.11 11.1
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
4.4.1.11 12.7
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
4.4.1.11 17.6
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
4.4.1.11 22.8
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
4.4.1.11 25.4
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
4.4.1.11 28.7
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes