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Literature summary extracted from

  • Kobayashi, J.; Yoshikane, Y.; Yagi, T.; Baba, S.; Mizutani, K.; Takahashi, N.; Mikami, B.
    Structure of 4-pyridoxolactonase from Mesorhizobium loti (2014), Acta Crystallogr. Sect. F, 70, 424-432.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.27 expressed in Escherichia coli B834 (DE3) cells Mesorhizobium loti

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.27 native and SeMet-labelled protein in complex with 5-pyridoxolactone, sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 50 mM ammonium citrate, pH 5.1 Mesorhizobium loti

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.27 5-Pyridoxolactone competitve inhibitor Mesorhizobium loti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.27 Zn2+ dependent on Mesorhizobium loti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.27 4-pyridoxolactone + H2O Mesorhizobium loti
-
 4-pyridoxate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.27 Mesorhizobium loti Q988B9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.27 Ni-NTA agarose column chromatography, hydroxyapatite column chromatography, and Shodex protein KW-803 gel filtration Mesorhizobium loti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.27 4-pyridoxolactone + H2O
-
 Mesorhizobium loti 4-pyridoxate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.27 homodimer x-ray crystallography Mesorhizobium loti