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Literature summary extracted from

  • Ahangar, M.S.; Vyas, R.; Nasir, N.; Biswal, B.K.
    Structures of native, substrate-bound and inhibited forms of Mycobacterium tuberculosis imidazoleglycerol-phosphate dehydratase (2013), Acta Crystallogr. Sect. D, 69, 2461-2467.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.19 expressed in Mycobacterium smegmatis strain mc24517 Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.19
-
 Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.19 3-Amino-1,2,4-triazole competitive Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.19 0.122
-
 D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate at pH 7.7 and 30°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.19 Mn2+ contains two Mn2+ ions, 0.05 mM Mn2+ used in assay conditions Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.19 570000
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.19 D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate Mycobacterium tuberculosis
-
 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.19 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.19 Ni-NTA affinity column chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.19 D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate
-
 Mycobacterium tuberculosis 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.19 tetradodecamer
-
 Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
4.2.1.19 HisB
-
 Mycobacterium tuberculosis
4.2.1.19 IGPD
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.19 1000
-
 D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate at pH 7.7 and 30°C Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.19 0.31
-
 3-Amino-1,2,4-triazole at pH 7.7 and 30°C Mycobacterium tuberculosis