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Literature summary extracted from

  • Podgorska, B.; Kujawska, G.; Skurzewski, M.; Batsko, O.; Kaczorowski, T.
    A rapid and simple method for detection of type II restriction endonucleases in cells of bacteria with high activity of nonspecific nucleases (2012), Acta Biochim. Pol., 59, 669-672.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.4 Mg2+ required Escherichia coli
3.1.21.4 Mg2+ required Klebsiella pneumoniae
3.1.21.4 Mg2+ required Citrobacter freundii
3.1.21.4 Mg2+ required Pseudescherichia vulneris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.4 double-stranded DNA + H2O Escherichia coli
-
 double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 double-stranded DNA + H2O Klebsiella pneumoniae
-
 double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 double-stranded DNA + H2O Citrobacter freundii
-
 double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 double-stranded DNA + H2O Pseudescherichia vulneris
-
 double-stranded DNA fragments with terminal 5'-phosphates
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.4 Citrobacter freundii
-
-
-
3.1.21.4 Escherichia coli
-
-
-
3.1.21.4 Klebsiella pneumoniae
-
-
-
3.1.21.4 Pseudescherichia vulneris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.4 phosphocellulose P-11 column chromatography, heparin Sepharose column chromatography, CM Sephadex C-50 gel filtration or Blue-agarose column chromatography Escherichia coli
3.1.21.4 phosphocellulose P-11 column chromatography, heparin Sepharose column chromatography, CM Sephadex C-50 gel filtration or Blue-agarose column chromatography Klebsiella pneumoniae
3.1.21.4 phosphocellulose P-11 column chromatography, heparin Sepharose column chromatography, CM Sephadex C-50 gel filtration or Blue-agarose column chromatography Citrobacter freundii
3.1.21.4 phosphocellulose P-11 column chromatography, heparin Sepharose column chromatography, CM Sephadex C-50 gel filtration or Blue-agarose column chromatography Pseudescherichia vulneris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.4 double-stranded DNA + H2O
-
 Escherichia coli double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 double-stranded DNA + H2O
-
 Klebsiella pneumoniae double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 double-stranded DNA + H2O
-
 Citrobacter freundii double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 double-stranded DNA + H2O
-
 Pseudescherichia vulneris double-stranded DNA fragments with terminal 5'-phosphates
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.4 BspI
-
 Citrobacter freundii
3.1.21.4 BstII
-
 Klebsiella pneumoniae
3.1.21.4 EcoRV
-
 Escherichia coli
3.1.21.4 EcoVIII
-
 Pseudescherichia vulneris
3.1.21.4 site-specific type II endonuclease
-
 Escherichia coli
3.1.21.4 site-specific type II endonuclease
-
 Klebsiella pneumoniae
3.1.21.4 site-specific type II endonuclease
-
 Citrobacter freundii
3.1.21.4 site-specific type II endonuclease
-
 Pseudescherichia vulneris
3.1.21.4 type II REase
-
 Escherichia coli
3.1.21.4 type II REase
-
 Klebsiella pneumoniae
3.1.21.4 type II REase
-
 Citrobacter freundii
3.1.21.4 type II REase
-
 Pseudescherichia vulneris
3.1.21.4 type II restriction enzyme
-
 Escherichia coli
3.1.21.4 type II restriction enzyme
-
 Klebsiella pneumoniae
3.1.21.4 type II restriction enzyme
-
 Citrobacter freundii
3.1.21.4 type II restriction enzyme
-
 Pseudescherichia vulneris