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Literature summary extracted from

  • Zhu, Y.; Wang, G.; Ni, H.; Xiao, A.; Cai, H.
    Cloning and characterization of a new manganese superoxide dismutase from deep-sea thermophile Geobacillus sp. EPT3 (2013), World J. Microbiol. Biotechnol., 30, 1347-1357.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 gene sod, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Geobacillus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 2-mercaptoethanol
-
 Geobacillus sp.
1.15.1.1 Ba2+ inhibitory at 1 mM Geobacillus sp.
1.15.1.1 Ca2+ inhibitory at 1 mM Geobacillus sp.
1.15.1.1 CHAPS
-
 Geobacillus sp.
1.15.1.1 Co2+ inhibitory at 1 mM Geobacillus sp.
1.15.1.1 Cu2+ 50% inhibition at 1 mM Geobacillus sp.
1.15.1.1 dithiothreitol
-
 Geobacillus sp.
1.15.1.1 EDTA strong inhibition at 10 mM Geobacillus sp.
1.15.1.1 Fe2+ inhibitory at 1 mM Geobacillus sp.
1.15.1.1 guanidine hydrochloride
-
 Geobacillus sp.
1.15.1.1 Mg2+ inhibitory at 1 mM Geobacillus sp.
1.15.1.1 additional information insensitivity of the recombinant enzyme to both KCN and H2O2 Geobacillus sp.
1.15.1.1 Ni2+ inhibitory at 1 mM Geobacillus sp.
1.15.1.1 phenylmethylsulfonyl fluoride
-
 Geobacillus sp.
1.15.1.1 SDS
-
 Geobacillus sp.
1.15.1.1 Triton X-100
-
 Geobacillus sp.
1.15.1.1 Tween 20
-
 Geobacillus sp.
1.15.1.1 Urea
-
 Geobacillus sp.
1.15.1.1 Zn2+ inhibitory at 1 mM Geobacillus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Mn2+ a Mn-SOD, Mn2+ activates, the manganese-binding site is formed by conserved residues His260, His308, Asp392, and His396 Geobacillus sp.
1.15.1.1 additional information no enzyme activity with Fe2+-reconstituted enzyme Geobacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 50230
-
 2 * 59000, SDS-PAGE, 2 * 50230, sequence calculation Geobacillus sp.
1.15.1.1 59000
-
 2 * 59000, SDS-PAGE, 2 * 50230, sequence calculation Geobacillus sp.
1.15.1.1 130700
-
 recombinant His-tagged enzyme, gel filtration Geobacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Geobacillus sp. T1T1K2 gene sod
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Geobacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 1204
-
 purified recombinant His-tagged enzyme, pH 7.8, 25°C Geobacillus sp.

Subunits

EC Number Subunits Comment Organism
1.15.1.1 homodimer 2 * 59000, SDS-PAGE, 2 * 50230, sequence calculation Geobacillus sp.
1.15.1.1 More modelling of the three-dimensional structure of SOD monomer, overview Geobacillus sp.

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 SOD
-
 Geobacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 25
-
 assay at Geobacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 50
-
 purified recombinant enzyme, stable Geobacillus sp.
1.15.1.1 80
-
 purified recombinant enzyme, 1 h, 64% activity remaining Geobacillus sp.
1.15.1.1 90
-
 purified recombinant enzyme, 1 h, 57% activity remaining Geobacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 7.8
-
 assay at Geobacillus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.15.1.1 5 11 purified recombinant enzyme, stable Geobacillus sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.15.1.1 Geobacillus sp. sequence calculation
-
 4.65

General Information

EC Number General Information Comment Organism
1.15.1.1 additional information Geobacillus sp. EPT3 SOD contains the LPPLPYRYDALEP sequence, which is identical to the conserved amino acid sequence (LPXLPYXXXXLEP) found at the N-terminal region of many Mn-SODs Geobacillus sp.